Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   MARCH 2014 , Volume 17 , Number 3; Page(s) 172 To 180.
 
Paper: 

IN SILICO ANALYSIS OF CHIMERIC TF, OMP31 AND BP26 FRAGMENTS OF BRUCELLA MELITENSIS FOR DEVELOPMENT OF A MULTI SUBUNIT VACCINE CANDIDATE

 
 
Author(s):  GHASEMI AMIR, RANJBAR REZA, AMANI JAFAR*
 
* APPLIED MICROBIOLOGY RESEARCH CENTER, BAQIYATALLAH MEDICAL SCIENCE UNIVERSITY, TEHRAN, IRAN. VANAK SQ. MOLASADRA ST. TEHRAN, IRAN
 
Abstract: 

Objective (s): Brucellosis, especially caused by Brucella melitensis, remains one of the most common zoonotic diseases worldwide with more than 500, 000 human cases reported annually. The commonly used live attenuated vaccine in ovine brucellosis prophylaxis is B. melitensis Rev1. But due to different problems caused by the administration of this vaccine, a protective subunit vaccine against B. melitensis is strongly demanded. Brucella BP26, Omp31 and TF proteins have shown a considerable potential as protective antigens for brucellosis. Chimeric proteins carrying epitopes or adjuvant sequences increase the possibility of eliciting a broad cellular or humoral immune response. In silico tools are highly suited to study, design and evaluate vaccine strategies.
Materials and Methods: In this study, a synthetic chimeric gene, encoding TF, BP26 93-111 and Omp3148-74 was designed. In order to predict the 3D structure of protein, modeling was carried out.
Results: Validation results showed that 91.1% of residues lie in favored or additional allowed region of Ramachandran plot. The epitopes in the chimeric protein are likely to induce both the B-cell and T-cell mediated immune responses.
Conclusion: The chimeric protein may be used as multi subunit for development of Brucella vaccine candidates.

 
Keyword(s): BRUCELLOSIS, CHIMERIC PROTEIN, EPITOPE, VACCINATION
 
 
References: 
  • Not Registered.
  •  
 
+ Click to Cite.
APA: Copy

GHASEMI, A., & RANJBAR, R., & AMANI, J. (2014). IN SILICO ANALYSIS OF CHIMERIC TF, OMP31 AND BP26 FRAGMENTS OF BRUCELLA MELITENSIS FOR DEVELOPMENT OF A MULTI SUBUNIT VACCINE CANDIDATE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 17(3), 172-180. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405542



Vancouver: Copy

GHASEMI AMIR, RANJBAR REZA, AMANI JAFAR. IN SILICO ANALYSIS OF CHIMERIC TF, OMP31 AND BP26 FRAGMENTS OF BRUCELLA MELITENSIS FOR DEVELOPMENT OF A MULTI SUBUNIT VACCINE CANDIDATE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2014 [cited 2021May18];17(3):172-180. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405542



IEEE: Copy

GHASEMI, A., RANJBAR, R., AMANI, J., 2014. IN SILICO ANALYSIS OF CHIMERIC TF, OMP31 AND BP26 FRAGMENTS OF BRUCELLA MELITENSIS FOR DEVELOPMENT OF A MULTI SUBUNIT VACCINE CANDIDATE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 17(3), pp.172-180. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405542.



 
  pdf-File
Yearly Visit 79
 
Latest on Blog
Enter SID Blog