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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   OCTOBER 2013 , Volume 16 , Number 10; Page(s) 1075 To 1087.
 
Paper: 

COMPARISON OF THE EX VIVO EXPANSION OF UCB-DERIVED CD34+IN 3D DBM/MBA SCAFFOLDS WITH USSC AS A FEEDER LAYER

 
 
Author(s):  HASHEMI ZAHRA SADAT, FOROUZANDEH MOGHADAM MEHDI*, SOLEIMANI MASOUD
 
* DEPARTMENT OF MEDICAL BIOTECHNOLOGY, FACULTY OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, INTERSECTION OF CHAMRAN AND AL-E-AHMAD HIGHWAYS, TEHRAN, IRAN
 
Abstract: 

Objective (s): Ex vivo expansion of hematopoitic stem cells is an alternative way to increase umbilical cord blood (UCB) -CD34+cells for bone marrow transplantation. For this purpose demineralized bone matrix (DBM) and mineralized bone allograft (MBA) as two scaffolds based on bone matrix and stem cell niche, were simultaneously used to enhance the effect of human mesenchymal progenitor cells (MPCs) - unrestricted somatic stem cells (USSCs) - as a feeder layer.
Materials and Methods: USSCs were isolated and characterized by morphological and immunological analysis then seeded on both scaffolds as a feeder layer. UCB-CD34+were isolated by MACS method and were co-culture expanded by USSC in 3D and 2D environments. After 3 weeks expansion, cells were counted and were assessed by karyotype, flow cytometry, clonogenic activity, and long-term culture-initiating cells (LTC-IC).
Results: Co-culture expansion in DBM and MBA was 29.22-fold and 27.77-fold, no significant differences in colony and LTC-IC were obtained. Maximum number of colonies belonged to the day 14 with the 73% CFU-GM (Colony Forming Unit- Granulocyte/Macrophage) in contrast to the day 0 which was BFU-E/CFU-E (Burst/Colony Forming Unit-Erythroid). Flow cytometry indicated that the percentage of CD34+marker was decreased in USSC co-culture and the highest percentage was observed in simple 2D culture.
Conclusion: Because of acid extraction in the DBM production process, mineral materials were removed and the protein background that was more flexible was presented. Therefore these results suggest that USSC-DBM can be a suitable ex vivo mimicry niche by intensifying of surface/volume ratio and supporting the stem cell differentiation and expansion.

 
Keyword(s): DBM, EX VIVO EXPANSION, MBA SCAFFOLD, UCB-CD34+, USSC CELLS
 
 
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APA: Copy

HASHEMI, Z., & FOROUZANDEH MOGHADAM, M., & SOLEIMANI, M. (2013). COMPARISON OF THE EX VIVO EXPANSION OF UCB-DERIVED CD34+IN 3D DBM/MBA SCAFFOLDS WITH USSC AS A FEEDER LAYER. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(10), 1075-1087. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405539



Vancouver: Copy

HASHEMI ZAHRA SADAT, FOROUZANDEH MOGHADAM MEHDI, SOLEIMANI MASOUD. COMPARISON OF THE EX VIVO EXPANSION OF UCB-DERIVED CD34+IN 3D DBM/MBA SCAFFOLDS WITH USSC AS A FEEDER LAYER. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2013 [cited 2021May18];16(10):1075-1087. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405539



IEEE: Copy

HASHEMI, Z., FOROUZANDEH MOGHADAM, M., SOLEIMANI, M., 2013. COMPARISON OF THE EX VIVO EXPANSION OF UCB-DERIVED CD34+IN 3D DBM/MBA SCAFFOLDS WITH USSC AS A FEEDER LAYER. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 16(10), pp.1075-1087. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405539.



 
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