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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   NOVEMBER 2013 , Volume 16 , Number 11; Page(s) 1163 To 1169.
 
Paper: 

THE EFFECT OF PLATELET RICH PLASMA ON CHONDROGENIC DIFFERENTIATION OF HUMAN ADIPOSE DERIVED STEM CELLS IN TRANSWELL CULTURE

 
 
Author(s):  MARDANI MOHAMMAD, KABIRI AZADEH, ESFANDIARI EBRAHIM, ESMAEILI ABOLGHASEM, POURAZAR ABBASALI, ANSAR MALEKMASSOUD, HASHEMIBENI BATOOL*
 
* DEPARTMENT OF ANATOMICAL SCIENCES AND MOLECULAR BIOLOGY, FACULTY OF MEDICINE, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Objective (s): Platelet-rich plasma (PRP) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. Little is known about the role of PRP as a promoter in chondrogenesis of human adipose derived stem cells (hADSCs). The aim of this study was to determine whether PRP may be considered as a natural and easy achievable source of growth factors to promote the chondrogenic differentiation of hADSCs in Transwell culture.
Materials and Methods: Biochemical, immunohistological and molecular assays were used to evaluate the effect of different concentrations (5%, 10%, and 15%) of PRP on chondrogenic differentiation of hADSCs in Transwell culture.
Results: The cells in the presence of 10% PRP produced markedly higher amounts of GAG and DNA, in comparison to the control group. PRP also increased chondrogenic markers in these cells, such as sox-9, aggrecan and collagen type II. A high expression level of collagen type X as a hypertrophic marker was observed in cartilage produced by using either PRP or TGF-
b1.
Conclusion: Our findings indicate that autologous PRP at an optimum concentration had beneficial effects on differentiation of hADSCs in Transwell culture. Further, in vivo studies are necessary to fully define the clinical implications of PRP.

 
Keyword(s): ADIPOSE-DERIVED STEM CELLS, PLATELET-RICH PLASMA, TRANSWELL CULTURE
 
 
References: 
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Click to Cite.
APA: Copy

MARDANI, M., & KABIRI, A., & ESFANDIARI, E., & ESMAEILI, A., & POURAZAR, A., & ANSAR, M., & HASHEMIBENI, B. (2013). THE EFFECT OF PLATELET RICH PLASMA ON CHONDROGENIC DIFFERENTIATION OF HUMAN ADIPOSE DERIVED STEM CELLS IN TRANSWELL CULTURE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(11), 1163-1169. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405512



Vancouver: Copy

MARDANI MOHAMMAD, KABIRI AZADEH, ESFANDIARI EBRAHIM, ESMAEILI ABOLGHASEM, POURAZAR ABBASALI, ANSAR MALEKMASSOUD, HASHEMIBENI BATOOL. THE EFFECT OF PLATELET RICH PLASMA ON CHONDROGENIC DIFFERENTIATION OF HUMAN ADIPOSE DERIVED STEM CELLS IN TRANSWELL CULTURE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2013 [cited 2021May12];16(11):1163-1169. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405512



IEEE: Copy

MARDANI, M., KABIRI, A., ESFANDIARI, E., ESMAEILI, A., POURAZAR, A., ANSAR, M., HASHEMIBENI, B., 2013. THE EFFECT OF PLATELET RICH PLASMA ON CHONDROGENIC DIFFERENTIATION OF HUMAN ADIPOSE DERIVED STEM CELLS IN TRANSWELL CULTURE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 16(11), pp.1163-1169. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405512.



 
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