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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   NOVEMBER 2013 , Volume 16 , Number 11; Page(s) 1139 To 1144.
 
Paper: 

PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTICOAGULANT FACTOR FROM THE SNAKE (ECHIS CARINATUS) VENOM

 
 
Author(s):  AMROLLAHI BYOKI ELHAM, ZARE MIRAKABADI ABBAS*
 
* DEPARTMENT OF VENOMOUS ANIMALS AND ANTIVENOM PRODUCTION, RAZI VACCINE AND SERUM RESEARCH INSTITUTE, KARAJ, IRAN
 
Abstract: 

Objective (s): Snake venoms contain complex mixture of proteins with biological activities. Some of these proteins affect blood coagulation and platelet function in different ways. Snake venom toxin may serve as a starting material for drug design to combat several pathophysiological problems such as cardiovascular disorders. In the present study, purification of anticoagulation factor from venom of snake (Echis carinatus) was studied. Anticoagulation activity of crude venom, fractions and purified peptide were determined by using prothrombin time (PT) and thrombin time (TT). Three fractions were partially purified from the venom of E. Carinatus by gel filtration on sephadex G-75 and final purification was performed by high-performance liquid chromatography (HPLC) with C18 column. A purified anticoagulant factor was derived which showed a single protein band in SDS-PAGE electrophoresis under reducing condition. Results of PT and TT tests for purified peptide (EC217) were found to be 102±4.242 and<5 min. respectively. Determination of molecular weight revealed that the active purified peptide (EC217) was about 30 KD. In conclusion, the present study showed that the venom of E. carinatus contains at least one anticoagulant factor.

 
Keyword(s): ANTICOAGULANT FACTOR, CHROMATOGRAPHY, ECHIS CARINATUS, SNAKE VENOM
 
 
References: 
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Click to Cite.
APA: Copy

AMROLLAHI BYOKI, E., & ZARE MIRAKABADI, A. (2013). PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTICOAGULANT FACTOR FROM THE SNAKE (ECHIS CARINATUS) VENOM. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(11), 1139-1144. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405483



Vancouver: Copy

AMROLLAHI BYOKI ELHAM, ZARE MIRAKABADI ABBAS. PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTICOAGULANT FACTOR FROM THE SNAKE (ECHIS CARINATUS) VENOM. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2013 [cited 2021May10];16(11):1139-1144. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405483



IEEE: Copy

AMROLLAHI BYOKI, E., ZARE MIRAKABADI, A., 2013. PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTICOAGULANT FACTOR FROM THE SNAKE (ECHIS CARINATUS) VENOM. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 16(11), pp.1139-1144. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405483.



 
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