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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   JANUARY 2014 , Volume 17 , Number 1; Page(s) 27 To 33.
 
Paper: 

COMPARISON OF THE LIVER FUNCTION AND HEPATIC SPECIFIC GENES EXPRESSION IN CULTURED MESENCHYMAL STEM CELLS AND HEPATOCYTES

 
 
Author(s):  NIKOOZAD ZAHRA, GHORBANIAN MOHAMMAD TAGHI*, REZAEI AREZOU
 
* SCHOOL OF BIOLOGY, DAMGHAN UNIVERSITY, DAMGHAN, IRAN
 
Abstract: 

Objective(s): Stem cell therapy is believed to be as a promising treatment strategy for tissue repair and regeneration. The plasticity specification of the adult stem cells, such as MSCs, has enabled that these cells to be used in the treatment of a broad spectrum of diseases like liver disorders. In this study, the production of urea and Albumin (Alb), glycogen storage, and expression of some liver genes including ?-fetoprotein (AFP), Alb, cytokeratin18 (CK18) and cytokeratin19 (CK19) was compared between mesenchymal stem cells (MSCs) and isolated rat hepatocytes.
Materials and Methods: The MSCs were isolated from rat femurs and tibias and cultured in ?-MEM, DMEM and RPMI mediums supplemented with serum. Hepatocytes were isolated from Rat livers and cultured in DMEM with serum. The expression of AFP, Alb, CK18, and CK19 genes was evaluated using the reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, the synthesis of albumin and urea of the cells was measured.
Results: In vitroconditions, MSCs and hepatocytes exhibited the characteristic functions of the liver such as capacity to synthesize Alb, urea, the storage of glycogen. In this study, the expression of some liver genes such as AFP, Alb, CK18 and CK19 at mRNA levels was also shown.
Conclusion: The results showed that MSCs exhibited some liver functions, and may be considered as an alternative source for adult stem cell transplantation in liver repair due to the excellent proliferation and differentiation capacities.

 
Keyword(s): ALBUMIN, GLYCOGEN, HEPATOCYTES, LIVER GENES, MESENCHYMAL STEM CELLS
 
 
References: 
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Click to Cite.
APA: Copy

NIKOOZAD, Z., & GHORBANIAN, M., & REZAEI, A. (2014). COMPARISON OF THE LIVER FUNCTION AND HEPATIC SPECIFIC GENES EXPRESSION IN CULTURED MESENCHYMAL STEM CELLS AND HEPATOCYTES. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 17(1), 27-33. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405479



Vancouver: Copy

NIKOOZAD ZAHRA, GHORBANIAN MOHAMMAD TAGHI, REZAEI AREZOU. COMPARISON OF THE LIVER FUNCTION AND HEPATIC SPECIFIC GENES EXPRESSION IN CULTURED MESENCHYMAL STEM CELLS AND HEPATOCYTES. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2014 [cited 2021May11];17(1):27-33. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405479



IEEE: Copy

NIKOOZAD, Z., GHORBANIAN, M., REZAEI, A., 2014. COMPARISON OF THE LIVER FUNCTION AND HEPATIC SPECIFIC GENES EXPRESSION IN CULTURED MESENCHYMAL STEM CELLS AND HEPATOCYTES. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 17(1), pp.27-33. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405479.



 
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