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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   AUGUST 2013 , Volume 16 , Number 8; Page(s) 936 To 941.
 
Paper: 

T LYMPHOCYTE APOPTOSIS IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS

 
 
Author(s):  RASTIN MARYAM*, MAHMOUDI MAHMOUD, HATEF MOHAMMADREZA, SAHEBARI MARYAM, TABASI NAFISEH, HAGHMORAD DARIUSH, NOSRATABADI REZA, ZAMANI SHAHRZAD, KHAZAEE MAHDIEH, MASOUDIAN MITRA
 
* IMMUNOLOGY RESEARCH CENTER, BUALI RESEARCH INSTITUTE, BUALI SQ., FERDOWSI SQ., MASHHAD, IRAN
 
Abstract: 

Objective(s): Apoptosis is a tightly regulated process and plays a crucial role in autoimmune diseases. Because abnormalities in apoptosis are considered to be involved in the pathogenesis of systemic lupus erythematosus (SLE), in present study we studied the apoptosis in T lymphocytes from Iranian SLE patientsat protein and gene expression levels for some molecules which are involved in apoptosis pathways.
Materials and Methods: Thirty five SLE patients (23 female, 12 male), and 20 age matched controls (10 female, 10 male) participated in this study. T lymphocytes were isolated from peripheral blood mononuclear cells (PBMCs) using MACS method. Apoptosis rate was studied at protein level by flow cytometer using Annexin V, and at gene expression level using semi-quantitative RT-PCR method for detection of Fas, FasL, Bcl-2, caspase 8, and caspase 9 genes.
Results: The percentage of apoptotic cells in SLE patients was not different in comparison with controls (20.2%±1.4 vs 21.1%±1.0), but the expression levels of FasL, caspase 8, and caspase 9 genes in all SLE patients and in female patients were significantly lower than controls; 0.45R vs 0.78R for FasL, 0.74R vs 1.0R for caspase 8, and 0.76R vs 1.26R for caspase 9 in all SLE patients and 0.37R vs 0.82R for FasL, 0.45R vs 1.6R for caspase 8, and 0.63R vs 1.56R for caspase 9 in female patients.
Conclusion: The expression levels of FasL, caspase 8 and caspase 9 molecules involved in apoptosis decreased in female, but not in male SLE patients.

 
Keyword(s): APOPTOSIS, AUTOIMMUNE, GENE EXPRESSION, SYSTEMIC LUPUSERYTHEMA TOSUS
 
 
References: 
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Click to Cite.
APA: Copy

RASTIN, M., & MAHMOUDI, M., & HATEF, M., & SAHEBARI, M., & TABASI, N., & HAGHMORAD, D., & NOSRATABADI, R., & ZAMANI, S., & KHAZAEE, M., & MASOUDIAN, M. (2013). T LYMPHOCYTE APOPTOSIS IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(8), 936-941. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405469



Vancouver: Copy

RASTIN MARYAM, MAHMOUDI MAHMOUD, HATEF MOHAMMADREZA, SAHEBARI MARYAM, TABASI NAFISEH, HAGHMORAD DARIUSH, NOSRATABADI REZA, ZAMANI SHAHRZAD, KHAZAEE MAHDIEH, MASOUDIAN MITRA. T LYMPHOCYTE APOPTOSIS IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2013 [cited 2021May12];16(8):936-941. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405469



IEEE: Copy

RASTIN, M., MAHMOUDI, M., HATEF, M., SAHEBARI, M., TABASI, N., HAGHMORAD, D., NOSRATABADI, R., ZAMANI, S., KHAZAEE, M., MASOUDIAN, M., 2013. T LYMPHOCYTE APOPTOSIS IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 16(8), pp.936-941. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405469.



 
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