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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   JULY 2014 , Volume 17 , Number 7; Page(s) 531 To 536.
 
Paper: 

EVALUATION OF HTLV-1 ACTIVITY IN HAM/TSP PATIENTS USING PROVIRAL LOAD AND TAX MRNA EXPRESSION AFTER IN VITRO LYMPHOCYTE ACTIVATION

 
 
Author(s):  YARI ATEFEH, REZAEE SEYYED ABDOLRAHIM, VALIZADEH NARGES, RAJAEE TARANEH, JAZAYERI SEYYED MOHAMMAD, SOLTANI MOJDEH, NOROUZI MEHDI*
 
* VIROLOGY DEPARTMENT, PUBLIC HEALTH SCHOOL, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Objective(s): HTLV-1 is the first human retrovirus that has been recognized and is associated with HAM/TSP and ATLL. Studies have shown that less than five percent of HTLV-1 infected carriers develop HAM/TSP or ATLL and about ninety-five percent remain asymptomatic. Therefore, the proviral load with Tax may affect cellular genes such as cytokines and oncogenes, as well as involve in pathogenicity.
Materials and Methods: Thirty HAM/TSP patients, thirty HTLV-1 healthy carriers, and MT-2 cell line were evaluated for HTLV-1 activity. PBMCs were isolated and activated using PMA and ionomycine. Real-time PCR and TaqMan methods were performed using specific primers and fluorescence probes for Tax expression and proviral load assessment. ?2microglobulin (
b2m) and albumin were used as controls in Tax expression and in proviral load, respectively.
Results: An insignificant increase in Tax expression was observed in rest PBMCs of HAM/TSP patients compared to healthy carriers. However, after lymphocyte activation there was a significant increase in Tax expression in HAM/TSP patients (P=0.042). The Proviral load in patients was significantly higher than in carriers. Moreover, there was a significant correlation between Tax mRNA expression in activated PBMCs and proviral load (R=0.37, P=0.012).
Conclusion: Although proviral load had been addressed as a valuable index for monitoring HTLV-1 infected subjects, the results of this study demonstrated that Tax expression in activated PBMCs along with proviral load assessment in HAM/TSP patients are a more reliable factor for determining the prognosis and monitoring healthy carriers and HAM/TSP patients.

 
Keyword(s): GENE EXPRESSION, HAM/TSP, HTLV-1, PROVIRAL LOAD, TAX MRNA
 
 
References: 
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APA: Copy

YARI, A., & REZAEE, S., & VALIZADEH, N., & RAJAEE, T., & JAZAYERI, S., & SOLTANI, M., & NOROUZI, M. (2014). EVALUATION OF HTLV-1 ACTIVITY IN HAM/TSP PATIENTS USING PROVIRAL LOAD AND TAX MRNA EXPRESSION AFTER IN VITRO LYMPHOCYTE ACTIVATION. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 17(7), 531-536. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405425



Vancouver: Copy

YARI ATEFEH, REZAEE SEYYED ABDOLRAHIM, VALIZADEH NARGES, RAJAEE TARANEH, JAZAYERI SEYYED MOHAMMAD, SOLTANI MOJDEH, NOROUZI MEHDI. EVALUATION OF HTLV-1 ACTIVITY IN HAM/TSP PATIENTS USING PROVIRAL LOAD AND TAX MRNA EXPRESSION AFTER IN VITRO LYMPHOCYTE ACTIVATION. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2014 [cited 2021May15];17(7):531-536. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405425



IEEE: Copy

YARI, A., REZAEE, S., VALIZADEH, N., RAJAEE, T., JAZAYERI, S., SOLTANI, M., NOROUZI, M., 2014. EVALUATION OF HTLV-1 ACTIVITY IN HAM/TSP PATIENTS USING PROVIRAL LOAD AND TAX MRNA EXPRESSION AFTER IN VITRO LYMPHOCYTE ACTIVATION. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 17(7), pp.531-536. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405425.



 
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