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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   MAY 2013 , Volume 16 , Number 5; Page(s) 675 To 682.
 
Paper: 

IDENTIFICATION OF NOVEL HYPOXIA RESPONSE GENES IN HUMAN GLIOMA CELL LINE A172

 
 
Author(s):  BAGHBANI FATEMEH, RAOOFIAN REZA, HASANZADEH NAZARABADI MOHAMMAD, HAMZEHLOEI TAYEBEH, SOUKHTANLOO MOHAMMAD, HEIDARI MANSUR, AFSHARZADEH SEYED MORTEZA, SHEKOUHI SAHAR, MORADI FAHIMEH, SARLI ABDOL AZIM, ZAVAR REZA JAVAD, MOJARRAD MAJID*
 
* FERDOWSI DEPARTMENT OF MEDICAL GENETICS, SCHOOL OF MEDICINE, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, IRAN
 
Abstract: 

Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA breaks in tumor cells. Identification of mechanisms responsible for these effects can lead to designing more efficient therapeutic strategies against tumor progression which results in improvement of patient prognosis.
Materials and Methods: In order to identify more hypoxia regulated genes which may have a role in glioblastoma progression, cDNA-AFLP was optimized as a Differential display method which is able to identify and isolate transcripts with no prior sequence knowledge.
Results: Using this method, the current study identified 120 Transcription Derived Fragments (TDFs) which were completely differentially regulated in response to hypoxia. By sequence homology searching, the current study could detect 22 completely differentially regulated known genes and two unknown sequence matching with two chromosome contig and four sequence matches with some Expressed Sequence Tags (ESTs).
Conclusion: Further characterizing of these genes may help to achieve better understanding of hypoxia mediated phenotype change in tumor cells.

 
Keyword(s): CDNA-AFLP, GLIOBLASTOMA, HYPOXIA
 
 
References: 
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Click to Cite.
APA: Copy

BAGHBANI, F., & RAOOFIAN, R., & HASANZADEH NAZARABADI, M., & HAMZEHLOEI, T., & SOUKHTANLOO, M., & HEIDARI, M., & AFSHARZADEH, S., & SHEKOUHI, S., & MORADI, F., & SARLI, A., & ZAVAR REZA, J., & MOJARRAD, M. (2013). IDENTIFICATION OF NOVEL HYPOXIA RESPONSE GENES IN HUMAN GLIOMA CELL LINE A172. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(5), 675-682. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405388



Vancouver: Copy

BAGHBANI FATEMEH, RAOOFIAN REZA, HASANZADEH NAZARABADI MOHAMMAD, HAMZEHLOEI TAYEBEH, SOUKHTANLOO MOHAMMAD, HEIDARI MANSUR, AFSHARZADEH SEYED MORTEZA, SHEKOUHI SAHAR, MORADI FAHIMEH, SARLI ABDOL AZIM, ZAVAR REZA JAVAD, MOJARRAD MAJID. IDENTIFICATION OF NOVEL HYPOXIA RESPONSE GENES IN HUMAN GLIOMA CELL LINE A172. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2013 [cited 2021May09];16(5):675-682. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405388



IEEE: Copy

BAGHBANI, F., RAOOFIAN, R., HASANZADEH NAZARABADI, M., HAMZEHLOEI, T., SOUKHTANLOO, M., HEIDARI, M., AFSHARZADEH, S., SHEKOUHI, S., MORADI, F., SARLI, A., ZAVAR REZA, J., MOJARRAD, M., 2013. IDENTIFICATION OF NOVEL HYPOXIA RESPONSE GENES IN HUMAN GLIOMA CELL LINE A172. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 16(5), pp.675-682. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405388.



 
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