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Journal:   INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY   OCTOBER-DESEMBER 2014 , Volume 8 , Number 3; Page(s) 325 To 332.
 
Paper: 

EFFECTS OF IN VITRO ZINC SULPHATE ADDITIVE TO THE SEMEN EXTENDER ON WATER BUFFALO (BUBALUSBUBALIS) SPERMATOZOA BEFORE AND AFTER FREEZING

 
 
Author(s):  DOROSTKAR KAMRAN, ALAVI SHOUSHTARI SAYED MORTAZA, KHAKI AMIR
 
* 
 
Abstract: 

Background: The objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and DNA stability) after cryopreservation. Materials and Methods: In this Prospective longitudinal laboratory study, semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions from Iran, Urmia during summer and autumn 2011, 25 samples were used in each treatment. Sperm progressive motility, viability and abnormal morphology were measured before and at 0.5 (T0), 1(T1) and 2(T2) hours after diluting semen(1:10 v/v) in Tris-citric acid based extender (without egg yolk and glycerol) at 37?C containing none (control group), 0.072, 0.144, 0.288, 0.576 and 1.152 mg/L zinc sulphate to investigate dose and time effects. Next, a Tris-citric acid-egg yolk-glycerol extender (20% egg yolk and 7% glycerol) containing the same amount of zinc sulphate was prepared, diluted semen (1:10 v/v) was cooled and kept into a refrigerated chamber (4?C) for 4 hours to equilibrate. Sperm progressive motility, viability, abnormal morphology, membrane integrity and DNA damage were estimated.The equilibrated semen was loaded in 0.5 ml French straws and frozen in liquid nitrogen. Later, the frozen semen was thawed and the same parameters as well as total antioxidant capacity (TAC) of the frozen-thawed semen were etermined.
Results: The results showed that zinc sulphate additive at the rate of 0.288 mg/L gave a higher protection of sperm progressive motility (53.7
±1.8% vs. 40.5±1.7%), viability (70.8±1.8% vs. 60.1±1.5%), membrane integrity (67.3±1.6% vs. 56.6±1.7%), DNA stability (10.1±0.47% vs. 11.8±0.33% damaged DNA) through the process of dilution, equilibration and freeze-thawing and caused a higher TAC level (81±3.3% vs. 63±3.2 ?mol/L) after freez-thawing compared to the control group. Adding 0.576 and 1.152 mg/L zinc sulphate, however, was deleterious to the sperm and significantly reduced the studied sperm parameters.
Conclusion: Adding 0.288 mg/L zinc sulphate to the extender, compared to the control group, gives a better sperm preservation upon freezing processes which in turn, may results in higher semen fertility. But, addition of higher zinc sulphate concentrations (0.576 and 1.152 mg/L) are detrimental to buffalo spermatozoa.

 
Keyword(s): SEMEN, ZINC, BUFFALO
 
 
References: 
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Click to Cite.
APA: Copy

DOROSTKAR, K., & ALAVI SHOUSHTARI, S., & KHAKI, A. (2014). EFFECTS OF IN VITRO ZINC SULPHATE ADDITIVE TO THE SEMEN EXTENDER ON WATER BUFFALO (BUBALUSBUBALIS) SPERMATOZOA BEFORE AND AFTER FREEZING. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 8(3), 325-332. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405367



Vancouver: Copy

DOROSTKAR KAMRAN, ALAVI SHOUSHTARI SAYED MORTAZA, KHAKI AMIR. EFFECTS OF IN VITRO ZINC SULPHATE ADDITIVE TO THE SEMEN EXTENDER ON WATER BUFFALO (BUBALUSBUBALIS) SPERMATOZOA BEFORE AND AFTER FREEZING. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY. 2014 [cited 2021May12];8(3):325-332. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405367



IEEE: Copy

DOROSTKAR, K., ALAVI SHOUSHTARI, S., KHAKI, A., 2014. EFFECTS OF IN VITRO ZINC SULPHATE ADDITIVE TO THE SEMEN EXTENDER ON WATER BUFFALO (BUBALUSBUBALIS) SPERMATOZOA BEFORE AND AFTER FREEZING. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, [online] 8(3), pp.325-332. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405367.



 
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