Paper Information

Journal:   INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY   OCTOBER-DESEMBER 2014 , Volume 8 , Number 3; Page(s) 333 To 340.
 
Paper: 

EXPRESSION OF RFAMIDE-RELATED PEPTIDE-3 (RFRP-3) MRNA IN DORSOMEDIAL HYPOTHALAMIC NUCLEUS AND KISS-1 MRNA IN ARCUATE NUCLEUS OF RAT DURING PREGNANCY

 
 
Author(s):  SABET SARVESTANI FATEMEH, TAMADON AMIN*, KOOHI HOSSEINABADI OMID, MOHAMMADI NEZHAD SAEED, RAHMANIFAR FARHAD, JAFARZADEH SHIRAZI MOHAMMAD REZA, TANIDEH NADER, MOGHADAM ALI, NIAZI ALI
 
* TRANSGENIC TECHNOLOGY RESEARCH CENTER, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SHIRAZ, IRAN
 
Abstract: 

Background: RFamide-related peptide-3 (RFRP-3) and kisspeptin (KiSS-1) are known to respectively inhibit and stimulate gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in rat. The aim of the present study was to evaluate the relative mRNA expression of RFRP-3 and KiSS-1 in the hypothalamus of pregnant rats. Materials and Methods: In a randomized controlled experimental study, the exact pregnancy day of 18 Sprague-Dawley rats were confirmed using the vaginal smear method and were equally assigned to three groups of days 7, 14 and 21 of pregnancy. Four nonpregnant female rats were ovariectomized and assigned as the control group. All rats were decapitated, and the dorsomedial hypothalamic nucleus (DMH) and the arcuate nucleus (ARC) for detection of KiSS-1 mRNA were separated from their hypothalamus to detect RFRP-3 and KiSS-1 mRNA respectively. Then, their relative expressions were compared between control and pregnant groups using real-time polymerase chain reaction (PCR).
Results: The relative expression of RFRP-3 mRNA in DMH did not change significantly during pregnancy (p>0.01). However, the relative expression of KiSS-1 mRNA in ARC was at its highest in day 7 of pregnancy and decreased until day 21 of pregnancy (p<0.01).
Conclusion: Decrease in GnRH and LH secretion during the pregnancy of rat may be controlled by constant expression of RFRP-3 mRNA and reduced expression of KiSS-1 mRNA in hypothalamus.

 
Keyword(s): KISS-1, RFAMIDE-RELATED PEPTIDE-3, PREGNANCY, DORSOMEDIAL HYPOTHALAMIC NUCLEUS, ARCUATE NUCLEUS
 
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