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Paper Information

Journal:   GOVARESH JOURNAL   FALL 2014 , Volume 19 , Number 3; Page(s) 175 To 181.
 
Paper: 

COMPARISON PCR AND CULTURE METHODS FOR DETECT COCCOID FORMS OF HELICOBACTER PYLORI

 
 
Author(s):  ALLAHKARAMI SOMAYEH*, SHAHHOSSEINY MOHAMMAD HASSAN, HAYATI ROODBARI NASIM, ESMAILI DAVOOD
 
* DEPARTMENT OF BIOLOGY, SCIENCE AND RESEARCH BRANCH, ISLAMIC AZAD UNIVERSITY,TEHRAN, IRAN
 
Abstract: 

Background: Helicobacter pylori (Pylori) was the most common cause of chronic gastritis and was linked to peptic ulcer disease and gastric cancer. Environmental factors such as water a reservoir of H.pylori which infect human. a Non-culture bacteria in coccoid forms widespread in aquatic environments.The objective of this study was elevating the diagnostic value of PCR and culture methods for for diagnosis coccoid forms of H.pylori.
Materials and Methods: To induce coccoid forms, ten different strains of H.pylori (H1-H10) were inoculated into 30 drinking water samples. Then, the samples were incubated at three different temperatures of 4oC, 22oC and 37oC for the durations of 30 and 60 days. The samples were cultured on brucella blood agar and DNA was also extracted also from them and PCR performed on samples.
Results: percentage of H.pylori cells detected at specified temperatures by the culture were 0%, 10% and 0% in the first month and were 0%,10%,30% in the second month whereas by the PCR molecular method were 30%, 80%, and 30% in the first month and were 20%, 20%, and 40% in the second month, respectively.
Conclusion: finding show PCR methodis more capable than culture fordetect the coccoid forms of H.pylori, therefore this method could be used to detect non- culture forms.

 
Keyword(s): HELICOBACTER PYLORI, COCCOID, PCR, CULTURE
 
 
References: 
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Citations: 
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APA: Copy

ALLAHKARAMI, S., & SHAHHOSSEINY, M., & HAYATI ROODBARI, N., & ESMAILI, D. (2014). COMPARISON PCR AND CULTURE METHODS FOR DETECT COCCOID FORMS OF HELICOBACTER PYLORI. GOVARESH JOURNAL, 19(3), 175-181. https://www.sid.ir/en/journal/ViewPaper.aspx?id=404366



Vancouver: Copy

ALLAHKARAMI SOMAYEH, SHAHHOSSEINY MOHAMMAD HASSAN, HAYATI ROODBARI NASIM, ESMAILI DAVOOD. COMPARISON PCR AND CULTURE METHODS FOR DETECT COCCOID FORMS OF HELICOBACTER PYLORI. GOVARESH JOURNAL. 2014 [cited 2021November27];19(3):175-181. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=404366



IEEE: Copy

ALLAHKARAMI, S., SHAHHOSSEINY, M., HAYATI ROODBARI, N., ESMAILI, D., 2014. COMPARISON PCR AND CULTURE METHODS FOR DETECT COCCOID FORMS OF HELICOBACTER PYLORI. GOVARESH JOURNAL, [online] 19(3), pp.175-181. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=404366.



 
 
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