Paper Information

Journal:   ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)   DECEMBER 2013 , Volume 15 , Number 12; Page(s) 28 To 31.
 
Paper: 

SHORT COMMUNICATION: PREVALENCE OF CAMPYLOBACTER JEJUNI AND COLI IN SHEEP CARCASSES BY USING CULTURAL AND PCR METHODS

 
 
Author(s):  SHAHROKHABADI REZA*, RAHIMI EBRAHIM, MOMMTAZ HASSAN, POURSAHEBI RAHELE
 
* YOUNG RESEARCHERS CLUB, SHAHR-E-KORD BRANCH, ISLAMIC AZAD UNIVERSITY, SHAHR-E-KORD, IRAN
 
Abstract: 

Background: Campylobacter species are common bacterial pathogens causing gastroenteritis in humans worldwide.
Materials and Methods: A total of 148 randomly sheep carcasses were sampled by surface section of neck meat taken immediately after slaughter analyzed using microbiological examinations.
Results: Campylobacter spp. was isolated from 10.13% meat cultures samples examined. Among these 80% sample were C. jejuni and 20% sample were C. coli. Using PCR assays, the number of positive campylobacters increased to 11.48%. Of these positive samples, 82.35% were C. jejuni and 17.65% were C. coli. Significantly higher prevalence rates of Campylobacter spp. (p<0.05) were found in the meat samples taken in summer (47.05%).
Conclusion: The PCR is a reliable and sensitive method which can be used as a diagnostic technique for the detection of campylobacter in lamb samples.

 
Keyword(s): CAMPYLOBACTER JEJUNI, CAMPYLOBACTER COLI, PCR, SHEEP
 
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