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Paper Information

Journal:   AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)   OCTOBER-DECEMBER 2014 , Volume 6 , Number 4; Page(s) 200 To 209.
 
Paper: 

EFFECTS OF TREATMENT WITH PLATINUM AZIDOTHYMIDINE AND AZIDOTHYMIDINE ON TELOMERASE ACTIVITY AND BCL-2 CONCENTRATION IN HEPATOCELLULAR CARCINOMA- INDUCED RATS

 
 
Author(s):  SABOKROUH ABDOLREZA, GOODARZI MOHAMAD TAGHI*, VAISI RAYGANI ASAD*, KHATAMI SHOHREH, TAGHIZADEH JAHED MASOUD
 
* DEPARTMENT OF CLINICAL BIOCHEMISTRY, SCHOOL OF MEDICINE, KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES, KERMANSHAH, IRAN
 
Abstract: 

Background: Telomerase activity increases in cancer cells. Bcl-2 is an antiapoptotic factor that its concentration grows in many cancer cells including hepatocellular carcinoma cells. In this study, an attempt was made to investigate the effects of a new synthetic compound, platinum azidothymidine (Pt-AZT) on treatment of rats with Hepatocellular Carcinoma (HCC) and to compare its effects with azidothymidine (AZT) in alteration of telomerase activity and Bcl-2 concentration in HCC.
Methods: Healthy adult male Wistar rats (n=100) were randomly divided into 4 groups (A, B, C, and D). Group A contained 25 healthy rats and was considered as the control group. Liver preneoplastic lesions were induced in remaining animals (n=75) using Solt-Farber resistant hepatocyte protocol. These animals were randomly allocated in groups B, C and D. Group B was negative control (untreated), groups C and D were treated by intraperitoneal injection (IP) of Pt-AZT (0.9 mg/kg/day) and AZT (0.3 mg/kg/day), respectively for 14 days. After the treatment period, telomerase activity and Bcl-2 concentration were determined in the rats’ liver.
Results: No HCC was developed in group A, but tumors were present in all other groups. Telomerase activity and Bcl-2 concentration were significantly lower in group C compared to groups B (0.159±0.06 vs.0.577±0.116 IU/L, p<0.001, respectively and 0.931±0.388 vs.3.94±0.74 ng/ml, p<0.001, respectively). Similar results were observed in comparison with group D (0.331±0.06 vs.0.577±0.116 IU/L, p<0.001, respectively and 0.931±0.388 vs.2.94±0.594 ng/ml, respectively). There was a significant negative correlation between telomerase activity and Bcl-2 concentration only in untreated cancer group (p=0.034).
Conclusion: In this study, higher anticancer activity of Pt-AZT in comparison to AZT was demonstrated. It effectively inhibits the growth of liver tumor in rats through extending apoptosis.

 
Keyword(s): HEPATOCELLULAR CARCINOMA, PLATINUM AZIDOTHYMIDINE, TELOMERASE ACTIVITY
 
 
References: 
 
Citations: 
 
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APA: Copy

SABOKROUH, A., & GOODARZI, M., & VAISI RAYGANI, A., & KHATAMI, S., & TAGHIZADEH JAHED, M. (2014). EFFECTS OF TREATMENT WITH PLATINUM AZIDOTHYMIDINE AND AZIDOTHYMIDINE ON TELOMERASE ACTIVITY AND BCL-2 CONCENTRATION IN HEPATOCELLULAR CARCINOMA- INDUCED RATS. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 6(4), 200-209. https://www.sid.ir/en/journal/ViewPaper.aspx?id=403206



Vancouver: Copy

SABOKROUH ABDOLREZA, GOODARZI MOHAMAD TAGHI, VAISI RAYGANI ASAD, KHATAMI SHOHREH, TAGHIZADEH JAHED MASOUD. EFFECTS OF TREATMENT WITH PLATINUM AZIDOTHYMIDINE AND AZIDOTHYMIDINE ON TELOMERASE ACTIVITY AND BCL-2 CONCENTRATION IN HEPATOCELLULAR CARCINOMA- INDUCED RATS. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB). 2014 [cited 2021October16];6(4):200-209. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=403206



IEEE: Copy

SABOKROUH, A., GOODARZI, M., VAISI RAYGANI, A., KHATAMI, S., TAGHIZADEH JAHED, M., 2014. EFFECTS OF TREATMENT WITH PLATINUM AZIDOTHYMIDINE AND AZIDOTHYMIDINE ON TELOMERASE ACTIVITY AND BCL-2 CONCENTRATION IN HEPATOCELLULAR CARCINOMA- INDUCED RATS. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), [online] 6(4), pp.200-209. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=403206.



 
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