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Paper Information

Journal:   MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS   2014 , Volume 3 , Number 2; Page(s) 75 To 84.
 
Paper: 

ANALYSIS OF NITRATE REDUCTASE MRNA EXPRESSION AND NITRATE REDUCTASE ACTIVITY IN RESPONSE TO NITROGEN SUPPLY

 
 
Author(s):  KAVOOSI GHOLAMREZA*, BALOTF SADEGH, ESHGHI HOMEIRA, HASANI HASAN
 
* INSTITUTE OF BIOTECHNOLOGY, SHIRAZ UNIVERSITY, SHIRAZ, IRAN
 
Abstract: 

Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite in the. The main objective of this research was to investigate stimulatory effects of sodium nitrate, potassium nitrate, ammonia and urea on the production/generation of the nitrate reductase mRNA in Triticum aestivum plants. The plants were grown in standard nutrient solution for 21 days and then starved in a media without nitrate for seven days. Starved plants were stimulated with various concentrations of sodium nitrate, potassium nitrate, ammonia and urea, and the expression of nitrate reductase mRNA was analyzed by real-time PCR. Our results indicated that starvation caused significant decrease in the production of nitrate reductase mRNA in the plant leaf. Sodium and potassium nitrate were capable of restoring the production of nitrate mRNA in a dose-dependent manner, since 50 mM of each produced the highest level of the mRNA. The stimulatory effect of potassium nitrate was higher than sodium nitrate, while ammonia and urea did not show such activity. At low concentrations, sodium nitrate and potassium nitrate caused significant increase in the nitrate/nitrite mRNA production, whereas high concentrations of these salts suppressed the expression of this gene considerably.

 
Keyword(s): TRITICUM AESTIVUM, NITRATE, POTASSIUM NITRATE, NITRATE REDUCTASE
 
 
References: 
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APA: Copy

KAVOOSI, G., & BALOTF, S., & ESHGHI, H., & HASANI, H. (2014). ANALYSIS OF NITRATE REDUCTASE MRNA EXPRESSION AND NITRATE REDUCTASE ACTIVITY IN RESPONSE TO NITROGEN SUPPLY. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 3(2), 75-84. https://www.sid.ir/en/journal/ViewPaper.aspx?id=403205



Vancouver: Copy

KAVOOSI GHOLAMREZA, BALOTF SADEGH, ESHGHI HOMEIRA, HASANI HASAN. ANALYSIS OF NITRATE REDUCTASE MRNA EXPRESSION AND NITRATE REDUCTASE ACTIVITY IN RESPONSE TO NITROGEN SUPPLY. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS. 2014 [cited 2021May07];3(2):75-84. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=403205



IEEE: Copy

KAVOOSI, G., BALOTF, S., ESHGHI, H., HASANI, H., 2014. ANALYSIS OF NITRATE REDUCTASE MRNA EXPRESSION AND NITRATE REDUCTASE ACTIVITY IN RESPONSE TO NITROGEN SUPPLY. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, [online] 3(2), pp.75-84. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=403205.



 
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