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Paper Information

Journal:   AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)   OCTOBER-DECEMBER 2014 , Volume 6 , Number 4; Page(s) 238 To 245.
 
Paper: 

INDUCTION OF STRONG AND SPECIFIC HUMORAL AND T-HELPER 1 CELLULAR RESPONSES BY HBSAG ENTRAPPED IN THE METHANOBREVIBACTER SMITHII ARCHAEOSOMES

 
 
Author(s):  AGHASADEGHI MOHAMMAD REZA, DELBAZ SEYED ALI, SADAT SEYED MEHDI, SIADAT SEYED DAVAR, SHAFIEE ARDESTANI MEHDI, RAHIMI POONEH, BOLHASSANI AZAM, VAHABPOUR ROUDSARI ROUHOLLAH, BAHRAMALI GOLNAZ, MOTEVALLI FATEMEH, DAVARI MEHDI, VAKILI HABIB, SHARIFAT SALMANI ALI*, BORHAN NOBARI MARYAM*
 
* DEPARTMENT OF HEPATITIS AND AIDS, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 

Background: Application of adjuvants with microbial origins is a recently highlighted approach in the vaccinology trials. Archaeosomes are among these microbial compounds with both adjuvant and liposomal activities and features.
Methods: In the present study, recombinant HBsAg encapsulated into Methanobrevibacter smithii (M. smithii) archaeosomes. Balb/c mice immunized with this compound and humoral and cytokine secretion pattern of immunized models analyzed.
Results: Frequency of IFN-
g secreting cells in the HBsAg-containing archaeosomes group was significantly higher than HBsAg and HBsAg+C/IFA groups (p?0.05). IgG2a titer in the sera of HBsAg-containing archaeosomes group was also significantly higher than this subclass titer in the other groups (p?0.05).
Conclusion: Analysis of induced responses revealed the immunopotentiating characteristics of M. smithii archaeosomes in the induction of T-helper 1 responses according to the dominance of IgG2a subtype and IFN-
g secreting splenocytes of immunized mice.

 
Keyword(s): CELLULAR, HEPATITIS B SURFACE ANTIGENS, HUMORAL, IMMUNITY, METHANOBREVIBACTER
 
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