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Paper Information

Journal:   WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES)   Spring 2004 , Volume 8 , Number 1; Page(s) 114 To 114.
 
Paper: 

POLYMORPHISM STUDY OF ESTERASE, PEROXIDASE AND MALATE DEHYDROGENASE ISOZYMES IN PISTACHIO CULTIVARS AND RELATED SPECIES

 
 
Author(s):  ALAMI A., TAEB M., LOTFI A., SADEGHIAN MOTAHAR S.Y.
 
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Abstract: 
Thirty genotypes of pistachio cultivars and related species were evaluated for genetic diversity using three polymorphic isozymes, i.e. Esterase, Peroxidase and Malate dehydrogenase. Young leaves of pistachio were crushed with extraction buffer containing: 20% sucrose, 0.01 M dithiothretiol, 2% polyethylene glycol, and 8% polyvenyl polypyrollidone. Samples were analyzed using isoelectric focussing on polyacrylamide gels containing 2% (WN) ampholyte. All the three isozymes revealed high degrees of polymorphism in pistachio cultivars and related species. Maximum polymorphism was observed for Est. enzyme. A total of 19 bands in Est. and 28 bands in MDH were observed in a wide range of pH gradient; however, in Per. there were 11 bands all of which located in a narrow range of pH gradient. Cluster analysis based on the three system enzymes revealed that all the 30 pistachio genotypes were in 8 main classes and 20 subclasses and the extent of genetic similarity reduced from cultivated varieties to species, which were finally classified in 3 groups. Sarakhs variety, a wild type of P. vera, was classified in a group between cultivated varieties and species.
 
Keyword(s): PISTACHIO, ISOZYME, ESTERASE, PEROXIDASE, MALATE DEHYDROGENASE, GENETIC DIVERSITY.
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

ALAMI, A., & TAEB, M., & LOTFI, A., & SADEGHIAN MOTAHAR, S. (2004). POLYMORPHISM STUDY OF ESTERASE, PEROXIDASE AND MALATE DEHYDROGENASE ISOZYMES IN PISTACHIO CULTIVARS AND RELATED SPECIES. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), 8(1), 114-114. https://www.sid.ir/en/journal/ViewPaper.aspx?id=3994



Vancouver: Copy

ALAMI A., TAEB M., LOTFI A., SADEGHIAN MOTAHAR S.Y.. POLYMORPHISM STUDY OF ESTERASE, PEROXIDASE AND MALATE DEHYDROGENASE ISOZYMES IN PISTACHIO CULTIVARS AND RELATED SPECIES. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES). 2004 [cited 2021November27];8(1):114-114. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=3994



IEEE: Copy

ALAMI, A., TAEB, M., LOTFI, A., SADEGHIAN MOTAHAR, S., 2004. POLYMORPHISM STUDY OF ESTERASE, PEROXIDASE AND MALATE DEHYDROGENASE ISOZYMES IN PISTACHIO CULTIVARS AND RELATED SPECIES. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), [online] 8(1), pp.114-114. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=3994.



 
 
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