The purpose of this research was to investigate the effect of glutamine supplement on changes in levels of plasma glutamine and immune factors in an athlete group. Subjects of the research were 20 wrestler, between aged 19 and 22, randomly divided in to control and test groups. They took part in training program of 16 sessions of exercise. The test group used the supplement and the control group used the placebo. Before the program the blood samples of subjects were taken to determine the level of plasma glutamine and concentration of louchocytes, lymphocytes and nutrophils. At the end of the first session of training and also before and after 16th session of training, blood samples were gathered. Subjects trained every day for 90 min and used the supplement or placebo at the end of the exercise. The collected data, were analyzed with ANOVA single factor (α=0.05). The result showed that the level of plasma glutamine after. The exercise in the 1st and 16th session reduced in control groups (P<0.01), but remind near to the rest level in test group. Changes of immune factors were similar in two groups. The rest level of glutamine at the end of training program reduced in control groups. The rest level of glutamine at the end of training program reduced in control group (P<0.001) and increased in test group. It does not appear that use of glutamine may prevent changes in immune factors. This finding does not reveal relation between reduced levels of plasma glutamine and immune factors.