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Paper Information

Journal:   RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)   2003 , Volume 8 , Number 3 (33); Page(s) 153 To 156.
 
Paper: 

ISOLATING A 68 KDA PROTEIN FRACTION OF LIZARD LEISHMANIA PROMASTIGOTE

 
 
Author(s):  KAZEMI B.*, HADADI Z.M., TAHVILDAR BIDROUNI F., GHADIRI A.
 
* Cellular and Molecular Biology Research Center, Shaheed Beheshti University of medical Sciences
 
Abstract: 

Background: Lizard leishmania has different specimen with variety of characteristics. Prior investigators have revealed that some lizard leishmania specimen could immunize balb-C rats against major leishmania. The present study was conducted to isolate a protein fraction of lizard leishmania to be used as vaccine against leishmana.
Materials and methods: A species of leishmana promastigote was grown up by inoculation of lizard cardiac blood on NNN medium and irrigated thrice by PBS. In order to prepare a prefect and effective vaccine, we isolated a 68 KDa protein fragment of lizard leishmania promastigote by using ammonium sulfate precipitation and gel filtration chromatography techniques.
Results: A protein band of almost 68 KDa was observed on ammonium sulfate precipitation. Then, it was passed through chromatography column containing Cephadex gel of G100 .Finally, the protein was dialyzed and the antibody was prepared in rabbit.
Conclusion: This protein has great antigenicity and could be used to develop a vaccine.

 
Keyword(s): LIZARD LEISHMANIA, ANTIGEN FRACTION, AMMONIUM SULFATE PRECIPITATION, GEL FILTERATION
 
References: 
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