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Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   June 2002 , Volume - , Number 53; Page(s) 79 To 85.
 
Paper: 

SEROTYPE IDENTIFICATION OF RECENT IRANIAN ISOLATES OF INFECTIOUS BRONCHITIS VIRUS BY TYPE-SPECIFIC MULTIPLEX RT-PCR

 
 
Author(s):  SEYFIABAD SHAPOURI M.R.*, MAYAHI M., CHARKHKAR S., SHAHRIARI ALI REZA
 
* MICROBIOLOGY DEPT., VETERINARY MEDICINE FACULTY, SHAHID CHAMRAN UNIVERSITY, AHWAZ, IRAN
 
Abstract: 

Fourteen isolates of infectious bronchitis virus (IBV) from Iran in 2001 were typed by a type-specific multiplex RT-PCR. The RT-PCR reaction has been designed to detect and differentiate strains of Massachusetts, D274 and 4/91 (793/B) types. Based on the DNA band produced in RT-PCR, twelve isolates were classified in the type Massachusetts and two isolates (13/2001 and 14/2001) in the type 4/91. The identity of isolate 14/2001, as being from the type 4/91, was also confirmed by sequence analysis of its RT-PCR product. The result of this study shows the presence of at least two types of IBV in Iran.

 
Keyword(s): INFECTIOUS BRONCHITIS VIRUS, RT-PCR, SEQUENCE, SEROTYPE
 
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