Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   June 2002 , Volume - , Number 53; Page(s) 57 To 65.
 
Paper: 

EFFECTS OF AFLATOXIN B1 ON LIVER NUCLEAR DNA BIOSYNTHESIS IN GROWING AND DAY-OLD CHICKENS

 
 
Author(s):  PARHIZKAR A., MIRHADI S.A., ALAMEH A.*
 
* BIOCHEMISTRY DEPT., FACULTY OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

The inhibitory effects of aflatoxin B1 (AFB1) on hepatic DNA biosynthesis was compared in growing and day-old chickens. A single ip dose of AFB1 (0, 25, 50,100 or 200mμg/kg BW) was administered to groups of then and day-old female Hybro-broiler chickens pre-treated with [3H]-thymidine. All the chickens were given labeled thymidine 22h prior to AFB1 administration and sacrificed 2h after AFB1 injection. Livers were removed and processed for DNA isolation and estimation of thymidine incorporation into nuclear DNA. A differential inhibitory effect of AFB1 on DNA biosynthesis in hen and chicks was noticed. The overall results showed that as compared to growing chickens, youngers are relatively more refractory to AFB1-induced inhibition in DNA biosynthesis. A single dose of AFB1 (25.50, 100 or 200 mμg/kg BW) administered to day-old chickens caused on average 22-28% inhibition in DNA biosynthesis. Whereas under similar conditions of dose and treatment to the growing group, a greater (36-47%) but dose-dependent inhibition in hens liver DNA is recorded.

 
Keyword(s): AFLATOXIN B1, AGE, CHICKEN, DNA BIOSYNTHESIS, LIVER
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 114
 
Latest on Blog
Enter SID Blog