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Paper Information

Journal:   IRANIAN BIOMEDICAL JOURNAL   July 2003 , Volume 7 , Number 3; Page(s) 113 To 118.
 
Paper: 

INHIBITORY EFFECTS OF AQUEOUS ONION AND GARLIC EXTRACTS ON GROWTH AND KERATINASE ACTIVITY IN TRICHOPHYTON MENTAGROPHYTES

 
 
Author(s):  *, RAZAFSHA M., SHAMS GHAHFAROKHI M.*,
 
* DEPT.OF MYCOLOGY AND DEPT. OF BIOCHEMESTRY, FACULTY OF MEDICAL SCIENCE, TARBIAT MODARES UNIVERSITY
 
Abstract: 
The effect of onion and garlic extracts on fungal growth and keratinolytic activity was ‎studied in Trichophyton mentagrophytes as one of the major etiologic agents of ‎human and animal dermatophytosis in Iran and other parts of the world. In order to ‎find out the best keratinase producer for further steps, culture conditions for 30 strains ‎of T. mentagrophytes isolated from human dermatophytosis were optimized on ‎specific solid and liquid media. All of the isolates produced the enzyme on both ‎selective culture media. The maximum keratinolytic activity at submerged cultivation ‎was reported for cultures of T. mentagrophytes isolate No. 1 grown for a 12-day ‎period at 32°C. Extracellular keratinase activity was in the range of 0.28 to 2.18 u/mg ‎protein in different isolates at predetermined optimal conditions. The growth of T. ‎mentagrophytes isolate No. 1 was inhibited in the presence of various concentrations ‎of onion and garlic extracts. This inhibition reached to a maximum of 100% for both ‎extracts at 10% v/v concentrations. Keratinase synthesis was also inhibited by two ‎extracts as a dose-dependent manner with maximums about 58.54 and 71.36 percent ‎at 5% concentrations, accordingly. In contrast to the fungal growth, keratinolytic ‎activity was inhibited more by garlic as compared with onion extract. This is the first ‎report on keratinase inhibition by these two natural compounds. Since fungal growth ‎and keratinolytic activity are important factors in pathogenesis of the dermatophytes, ‎their inhibition by onion and garlic indicate that these substances may have potential ‎values for treatment of human and animal dermatophytosis‏.‏
 
Keyword(s): TRICHOPHYTON MENTAGROPHYTES, KERATINASE, ONION, GARLIC, DERMATOPHYTOSIS
 
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