Paper Information

Journal:   IRANIAN BIOMEDICAL JOURNAL   July 2003 , Volume 7 , Number 3; Page(s) 91 To 98.
 
Paper: 

MUTAGENESIS OF XANTHOMONAS CAMPESTRIS AND SELECTION OF STRAINS WITH ENHANCED XANTHAN PRODUCTION

 
 
Author(s):  , MEHRGAN H.*, MAZAHERI ASADI MAHNAZ, MORTAZAVI SEYED ALI REZA
 
* 
 
Abstract: 
Xanthan gum is a microbial polysaccharide of great commercial importance as it has ‎unusual rheological properties in solution and consequent range of applications. In ‎this study, a series of mutants were isolated from Xanthomonas campestris PTCC ‎‎1473 by ethyl methanesulfonate mutagenesis. The polysaccharide yield of one mutant, ‎XC1473E2, was 30% better than that of the parent strain. It also showed higher ‎Xanthan formation and glucose consumption rates compared to the parent strain. ‎Xanthan produced by the mutant had enhanced viscosity, higher pseudoplasticity and ‎larger molecular weight. Since mutant XC1473E2 appeared white on agar plates, it ‎underwent pigment extraction with methanol. Contrary to the Parent strain, the mutant showed no absorption at 443nm, i.e. the wavelength related ‎to yellow pigment. This finding suggested that yellow pigmentation and normal ‎Xanthan biosynthesis are not necessarily concurrent. In general, mutant XC1473E2 ‎seems to be a strain with interesting characteristics for use in commercial production ‎of Xanthan‏.‏
 
Keyword(s): XANTHAN, XANTHOMONAS CAMPESTRIS, MUTATION, XANTHOMONADIN
 
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