Paper Information

Journal:   PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)   SPRING 2010 , Volume 8 , Number 1; Page(s) 7 To 13.
 
Paper: 

THE EFFECT OF PROTEIN AND DNA FRACTION OF TOXOPLASMA GONDII ON NITRIC OXIDE PRODUCTION AND GROWTH AND SURVIVAL OF PERITONEAL MACROPHAGES

 
 
Author(s):  DANESHMANDI S.*, HAJIMORADI M., ROUDBARY M., AMARI A.
 
* DEPARTMENT OF IMMUNOLOGY, SCHOOL OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Introduction
Toxoplasma gondii is an obligate intracellular parasite that invades a wide variety of host cells including macrophages and survives within it. The parts of Toxoplasma that participate in the mechanism of its evasion from immune system and macrophage defenses are not completely defined. In this study, we evaluated the effect of protein and DNA Toxoplasma fractions on proliferation and nitric oxide production by peritoneal macrophages.
Material and Methods
The viability of macrophages was evaluated using3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) reduction assay and the production of nitrite using Griess method.
Results
MTT reduction and hence the growth and viability of macrophages in a dose of 200 ng/ml was significantly lower than those of the negative control (P=0.022); in other lower doses of protein it was not statistically significant (P>0.05). Different doses of Toxoplasma protein fraction did not affect NO production (P>0.05). MTT assay and NO production in different doses of DNA fraction was not different (P>0.05).
Conclusion
According to the results of the present study, protein fraction of Toxoplasma has a suppressive effect on macrophage viability, but this effect is dose dependent. Protein fraction of Toxoplasma does not affect the amount of NO production by macrophage. The isolated DNA fraction of Toxoplasma did not influence the viability and NO production of macrophages. So, the ability of evasion of Toxoplasma gondii from macrophage defense is due to a component of its protein.

 
Keyword(s): TOXOPLASMA GONDII, MACROPHAGE, NITRIC OXIDE, MTT ASSAY
 
References: 
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