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Paper Information

Journal:   PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)   SPRING 2010 , Volume 8 , Number 1; Page(s) 1 To 6.
 
Paper: 

A MOLECULAR EPIDEMIOLOGY SURVEY OF CUTANEOUS LEISHMANIASIS IN PATIENT REFERRING TO PARASITOLOGY LAB AT SHIRAZ SCHOOL OF MEDICINE AND THE IMPORTANCE OF PCR ASSAY

 
 
Author(s):  FAKHAR M.*, MIKAEILI F., HATAM G.R., HABIBI P., KARAMIAN M., MOTAZEDIAN M.H., BANIMOSTAFAVI E.
 
* DEPARTMENT OF PARASITOLOGY AND MYCOLOGY, SCHOOL OF MEDICINE, MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, SARI, IRAN
 
Abstract: 

Introduction
Fars Province is one of endemic foci of cutaneous leishmaniasis (CL). The present study aimed to conduct a molecular epidemiology survey of patients referring to Parasitology Lab at Shiraz School of Medicine and evaluate the PCR assay for the diagnosis of CL.
Material and Methods
This retrospective study was carried out for patients referring to Parasitology department at Shiraz School of Medicine, during 1380-84. The disease was diagnosed by direct smear and staining with Giemsa; if the direct smear was negative, specific PCR was done on the DNA extracted from the direct smear to genus and species identification and culture. The data were registered and analyzed by SPSS software.
Results
Of 186 suspected patients, 104 (56%) cases were infected with CL. PCR results were positive in 35 (56.4%) out of 62 smears, among which microscopic examination did not reveal Leishmania amastigotes. Also, Leishmania species isolated from the patients residing in the urban and rural areas were Leishmania tropica and Leishmania major by species-specific primers, respectively.
Conclusion
Since in some cases the direct smear is reported negative, we suggest using PCR methods on smears in endemic regions.

 
Keyword(s): CUTANEOUS LEISHMANIASIS, MOLECULAR EPIDEMIOLOGY, DEMOGRAPHIC, LEISHMANIA MAJOR, LEISHMANIA TROPICA
 
References: 
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