Paper Information

Journal:   AGRICULTURAL RESEARCH   2007 , Volume 7 , Number 3; Page(s) 155 To 164.
 
Paper: 

DETERMINATION OF SUITABLE POLLINIZERS FOR SWEET CHERRY (PRUNUS AVIUM) CULTIVAR 'SORATI-E-HAMEDAN' USING ALLELE-SPECIFIC PCR

 
 
Author(s):  ERSHADI A., DASHTI F., HASANI MOGHADAM E.
 
* 
 
Abstract: 
Self-incompatibility in sweet cherry is attributed to a multi-allelic locus, S-locus, controlled gametophytically. In commercial sweet cherry orchards, compatible pollinizers must be planted to set an economic crop. In this study, suitable pollinizers for cultivar 'Sorati-e-Harnedan' were determined based on S-allele genotyping, with allele-specific primers, as well as flowering date coincidence of seven sweet cherry cultivars 'Mojtahedi', 'Hajyosoufi', 'Shoa-al- Saltaneh', 'Ghermez-e- Rezayieh', 'Siah-e-Qazvin, 'Siah-e- Mashhad' and 'Shabestar'. Using 13 allele specific primer pair's, two cross-incompatiblity groups (CIGs) S3S4 and S4S14 were identified.
'Sorati-e-Hamaden' and 'Siah-e-Qazvin' cultivars had S4S14 genotype, and were completely cross incompatible. S-genotype of 'Mojtahedi', 'Haj Yosoufi', 'Shoa-al-Saltaneh' and 'Ghermez-e- Rezayieh' cultivars were S3S4 are semi-compatible with 'Sorati-e-Hamedan'. In 'Siah-e-Mashhad' and 'Shabestar' only S3 allele identified and these cultivars were fully compatible with 'Sorati-e- Hamedan'. All cultivars except 'Shoa-al-Saltaneh' had enough flowering date coincidence (
³75) with 'Sorati-e-Hamedan'. Based on the results obtained, 'Shabestar', 'Siah-e-Mashhad', 'Mojtahdi' and 'Germez-e-Rezayieh' cultivars were considered as compatible and suitable pollinizers following by 'Hajyosoufi'. This study showed that, specific amplification of S-alleles by polymerase chain reaction is a rapid and useful approach for determination the cross-incompatibility groups and selecting the compatible pollinizers.
 
Keyword(s): SWEET CHERRY, SELF-INCOMPATIBILITY, CROSS-INCOMPATIBILITY, POLLINIZER, POLYMERASE CHAIN REACTION
 
References: 
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