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Paper Information

Journal:   JOURNAL OF MASHHAD DENTAL SCHOOL   SPRING 2012 , Volume 36 , Number 1 (80); Page(s) 79 To 90.
 
Paper: 

IN VITRO HISTOLOGICAL INVESTIGATION OF INTERACTIONS BETWEEN RAT’S MESENCHYMAL STEM CELLS AND HUMAN GINGIVAL MATRIX

 
 
Author(s):  YARJANLI ZAHRA, MAHDAVI SHAHRI NASSER*, MOGHADDAM MATIN MARYAM, FEREIDONI MASOUD, BANIHASHEMRAD SEYEDALI
 
* DEPT OF BIOLOGY, STEM CELL RESEARCH GROUP, INSTITUTE OF BIOTECHNOLOGY, SCHOOL OF SCIENCE, FERDOWSI UNIVERSITY OF MASHHAD, MASHHAD, IRAN
 
Abstract: 
Introduction: Extracellular matrix of natural tissues can be used as a scaffold for reconstructing biological tissues and organs. In this study, decellularized human gingival matrix was used as a scaffold for investigating the interactions of rat’s bone marrow mesenchymal stem cells with human gingival matrix.
Materials & Methods: To reach this goal, human gingival tissues were decellularized by two detergents sodium dodecyl sulfate (SDS) and Triton X-100. After washing and sterilization procedures, scaffolds were divided into 3 groups. Low density (LD) group was cultivated with 8x104 cells /cm2, high density (HD) group was cultivated with 8x105 cells/cm2, and control (C) group, was maintained in culture medium without any cells. Microscopic sections were prepared from the scaffolds before and after 1, 2 and 4 weeks of culture with mesenchymal cells and were stained with Hematoxylin-Eosin.
Repeated measure ANOVA was used to study the cell density alteration significance within the matrixes and post tests of means comparisons were performed within and between the groups. Also, gingival samples before and after decellularization procedure were investigated by scanning electron microscopy.
Results: Histological study of decellularized scaffolds revealed that nuclear and cellular components of the tissues were completely removed. Scanning electron microscopy of the scaffolds indicated that collagen fibers of connective tissue remained intact. Study of the scaffolds 1, 2 and 4 weeks after culture, revealed penetration of mesenchymal stem cells in scaffold, migration of cells towards connective tissue’s papilla, and moreover epithelium-like structures. Statistical analysis indicated that cell density in HD group was significantly (P<0.05) higher than LD group. Cell density in both LD and HD groups significantly increased at 2nd week and decreased after4 weeks of culture.
Conclusion: According to the results, scaffolds prepared from human gingival matrix can be a suitable scaffold for studying In vitro cell behaviors during oral wound healing.
 
Keyword(s): EXTRACELLULAR MATRIX, EPITHELIUM, INTERACTION, SCAFFOLD, HUMAN GINGIVA, MESENCHYMAL STEM CELLS
 
 
References: 
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Citations: 
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APA: Copy

YARJANLI, Z., & MAHDAVI SHAHRI, N., & MOGHADDAM MATIN, M., & FEREIDONI, M., & BANIHASHEMRAD, S. (2012). IN VITRO HISTOLOGICAL INVESTIGATION OF INTERACTIONS BETWEEN RAT’S MESENCHYMAL STEM CELLS AND HUMAN GINGIVAL MATRIX. JOURNAL OF MASHHAD DENTAL SCHOOL, 36(1 (80)), 79-90. https://www.sid.ir/en/journal/ViewPaper.aspx?id=277304



Vancouver: Copy

YARJANLI ZAHRA, MAHDAVI SHAHRI NASSER, MOGHADDAM MATIN MARYAM, FEREIDONI MASOUD, BANIHASHEMRAD SEYEDALI. IN VITRO HISTOLOGICAL INVESTIGATION OF INTERACTIONS BETWEEN RAT’S MESENCHYMAL STEM CELLS AND HUMAN GINGIVAL MATRIX. JOURNAL OF MASHHAD DENTAL SCHOOL. 2012 [cited 2021July26];36(1 (80)):79-90. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=277304



IEEE: Copy

YARJANLI, Z., MAHDAVI SHAHRI, N., MOGHADDAM MATIN, M., FEREIDONI, M., BANIHASHEMRAD, S., 2012. IN VITRO HISTOLOGICAL INVESTIGATION OF INTERACTIONS BETWEEN RAT’S MESENCHYMAL STEM CELLS AND HUMAN GINGIVAL MATRIX. JOURNAL OF MASHHAD DENTAL SCHOOL, [online] 36(1 (80)), pp.79-90. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=277304.



 
 
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