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Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   DECEMBER 2010 , Volume 28 , Number 112; Page(s) 607 To 620.
 
Paper: 

EFFECT OF TRANSFORMING GROWTH FACTOR- β3 AND BONE MORPHOGENETIC PROTEIN-6 GROWTH FACTORS ON CHONDROGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS IN ALGINATE SCAFFOLD

 
 
Author(s):  HASHEMIBENI BATOOL, RAZAVI SHAHNAZ*, ESFANDIARY EBRAHIM, KARBASI SAEED, MARDANI MOHAMMAD, SADEGHI FARZANEH, NASRE ESFAHANI MOHAMMADHOSEIN, NADALI FATEMEH, SHAFIEZADE HAMID
 
* DEPARTMENT OF ANATOMICAL SCIENCES, SCHOOL OF MEDICINE. ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISTAHAN, IRAN
 
Abstract: 

Background: Cartilage damages and diseases such as osteoarthritis are rather worldwide problems of many people. In addition, articular cartilage has a limited ability to repair. Current treatment methods for cartilage tissue injuries lead to formation of fibrous tissue, apoptosis, and further cartilage degeneration. Therefore, engineering of cartilage tissue with adult stem cells is considered necessary. This study aimed to investigate the effect of TGF-b3 and BMP-6 growth factors on chondrogenic differentiation of adiposederived stem cells in alginate scaffold.
Methods: Stem cells were isolated from human subcutaneous adipose tissue with collagenase enzyme digestion. The isolated cells were treated with chondrogenic medium supplemented with TGF-
b3 and BMP-6 on alginate scaffold then harvested after 3 weeks. Histological, immunohistochemical and RTPCR methods were performed for assessment of cartilage-specific markers.
Findings: Our results revealed that chondrogenesis of ADSCs on alginate scaffold induced by TGF-
b3 and BMP-6 growth factors. Immunohistochemical method was shown deposition of typical cartilage extracellular matrix components such as type IT collagen and aggrecan in constructs. RT-PCR analysis of cartilage-specific genes, also confirmed the induction of the chondrocytic phenotype upon stimulation with TGF-b3 and BMP-6. Also, TGF-b3 and BMP-6 together increased the expression of aggrecan gene in compare with one growth factor alone.
Conclusion: It can be concluded that TGF-
b3 and BMP-6 promote chondrogenesis of ADSCs in 3-D culture and the use of both growth factors together increase the expression of aggrecan gene in compare with one growth factor alone

 
Keyword(s): ADIPOSE-DERIVED STEM CELLS, ALGINATE, TISSUE ENGINEERING OF CARTILAGE, BMP-6, TGF-β3
 
References: 
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