Paper Information

Journal:   JOURNAL OF CROP BIOTECHNOLOGY   FALL 2011 , Volume 1 , Number 1; Page(s) 13 To 21.
 
Paper:  MICROSPORE EMBRYOGENESIS IN DIFFERENT HYBRIDS OF BREAD WHEAT (TRITICUM AESTIVUM L.) AND IMPROVEMENT OF EMBRYOGENESIS EFFICIENCY VIA 2,4-D STRESS PRE-TREATMENT
 
Author(s):  TAYEFE AFSHARI B., SHARIATPANAHI M.E.*, VAHABZADEH M.
 
* DEPARTMENT OF TISSUE CULTURE AND GENE TRANSFORMATION, AGRICULTURAL BIOTECHNOLOGY RESEARCH INSTITUTE OF IRAN, MAHDASHT ROAD, KARAJ
 
Abstract: 

The objective of this study was to achieve improved induction of embryogenesis in bread wheat microspores. Some F1 hybrids i.e M85-6×90, M85-8×90, mv17×shiroudi, mv17×kavir and kavir×bam were used. Microspores were cultured in A2 medium containing different amounts of maltose (60 & 90 g/lit) depending on the genotype used. Analysis of variance for embryogenesis and regenerable embryos showed a highly significant difference between hybrids but there was no significant difference between the media (A2-60 & A2-90) and no interaction effects. M85-6×90, mv17×Shiroudi and mv17×kavir produced the highest ratio of embryogenesis among the hybrids. In the regeneration phase, mv17×Shiroudi and M85-6×90 had the highest frequency of regenerable embryos. The effect of 2,4-D as a novel inducer of microspores embryogenesis in Falat was investigated. Falat is known as the most responsive wheat cultivar to microspore culture technology,. Microspores were subjected to 2,4-D at 3 different concentrations of 15, 25, 35 mg/l for 30 minutes while microspores without any stress treatment were used as the control. The embryogenesis of microspores stressed with 2,4-D were better than that of the control. The highest embryogenesis yield was achieved at 15 mg/l 2,4-D. The most regenerated embryos were obtained at 2,4-D concentrations of 15 and 25 mg/l. According to the results obtained, 2,4-D is introduced as a new embryogenesis inducer in microspores of wheat.

 
Keyword(s): WHEAT, MICROSPORE, EMBRYOGENESIS, DOUBLED HAPLOID, 2,4-D (2,4-DI CHLOROPHENOXYACETIC ACID)
 
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