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Paper Information

Journal:   JOURNAL OF CROP BIOTECHNOLOGY   FALL 2011 , Volume 1 , Number 1; Page(s) 1 To 11.
 
Paper: 

PROTEOMIC ANALYSIS OF ROOT AND LEAF IN RICE UNDER SALINITY STRESS

 
 
Author(s):  HOSSEINI SALKDEH GH.*, NASRABADI D.
 
* DEPARTMENT OF GENOMICS, AGRICULTURAL BIOTECHNOLOGY RESEARCH INSTITUTE OF IRAN (ABRII), KARAJ, IRAN
 
Abstract: 

Rice is currently the main staple food for more than 2 billion people in Asia, Africa and Latin America. It is also an excellent model cereal for molecular biology and genetics research. Salinity is a major factor limiting rice production worldwide. The analysis of stress-responsiveness in plants is an important route to the discovery of genes conferring stress tolerance which could be then uses in breeding programs. To further understand the mechanism of plant response to salinity, we employed a proteomic approach to profile the protein changes of the third leaf of rice and root under salt stress. Plants were grown in Yoshida nutrient solution and salt was stress imposed after 25 days. Plants were treated by 100 mM NaCl for10 Days. After that, third leaves and total root were collected from the control and salt stressed plants. The Na+ and K+ content of the leaves/roots and several yield components changed significantly in response to short-term salt stress and their proteome patterns were analyzed using 2-DE in triplicates. The expression pattern of proteins changed in all the leaves/roots in response to stress considerably. More than 488 and 345 proteins were detected repeatedly in the 2D gels for root and leaf, respectively. 107 proteins in the root and 86 proteins in the leaf of two genotypes showed significant response to stress. Three proteins in leaf gels and 2 proteins in root gels were selected and identified by ESI-Q-TOF. The most important ones included ferritin, rubisco activase and ascorbat peroxidase in leaf and peroxidase and ascorbat peroxidase in root. All of those were enzymes and involved in detoxification and removal of reactive oxygen species (peroxidase, ascorbat peroxidase), iron homeostasis (ferritin) or were involved in the activation of other enzymes (rubisco activase).

 
Keyword(s): RICE, ORYZA SATIVA, SALINITY, PROTEOMICS, 2D, OXIDATIVE STRESS
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

HOSSEINI SALKDEH, G., & NASRABADI, D. (2011). PROTEOMIC ANALYSIS OF ROOT AND LEAF IN RICE UNDER SALINITY STRESS. JOURNAL OF CROP BIOTECHNOLOGY, 1(1), 1-11. https://www.sid.ir/en/journal/ViewPaper.aspx?id=276926



Vancouver: Copy

HOSSEINI SALKDEH GH., NASRABADI D.. PROTEOMIC ANALYSIS OF ROOT AND LEAF IN RICE UNDER SALINITY STRESS. JOURNAL OF CROP BIOTECHNOLOGY. 2011 [cited 2021July30];1(1):1-11. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=276926



IEEE: Copy

HOSSEINI SALKDEH, G., NASRABADI, D., 2011. PROTEOMIC ANALYSIS OF ROOT AND LEAF IN RICE UNDER SALINITY STRESS. JOURNAL OF CROP BIOTECHNOLOGY, [online] 1(1), pp.1-11. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=276926.



 
 
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