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Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCE (UNIVERSITY OF TABRIZ)   SUMMER 2012 , Volume 22 , Number 2; Page(s) 67 To 78.
 
Paper: 

MOLECULAR DETECTION OF POTATO VIRUS Y USING UNIVERSAL PRIMERS FROM ARDABIL PROVINCE

 
 
Author(s):  GHASEMZADEH A.*, SOKHANDANBASHIR N., KHAKVAR R.
 
* DEPT OF PLANT PROTECTION FACULTY OF AGRICULTURE, THE UNIVERSITY OF TABRIZ, IRAN
 
Abstract: 

Potato virus Y (PVY) is the most important virus that infects Solanaceae family worldwide. In order to detect potyviruses from Solanaceae in East Azarbaijan and Ardabil provinces, 112 samples with mosaic, mottling and yellowing symptoms were collected. Then, the samples were checked by Reverse transcription polymerase chain reaction (RT-PCR) with Potyvirus universal primers which ended up in detection of the virus from 23 samples. In order to identify PVY, some of these samples with mosaic and yellowing symptoms were selected for cloning and sequencing. Finally one of them was recognized as PVY. For determination of taxonomic position of PVY isolate from Iran, the sequence data from Iranian PVY was aligned and compared with the related 165 isolates from Gene Bank. Results of phylogenetic analyses showed that PVY isolates were grouped in 5 clusters wherein the Iranian PVY isolate was placed in cluster 1 together with isolates from America, Canada, Syria, France, England, China and Poland.

 
Keyword(s): POTATO, POTATO VIRUS Y, POTYVIRUS, UNIVERSAL PRIMERS
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

GHASEMZADEH, A., & SOKHANDANBASHIR, N., & KHAKVAR, R. (2012). MOLECULAR DETECTION OF POTATO VIRUS Y USING UNIVERSAL PRIMERS FROM ARDABIL PROVINCE. JOURNAL OF AGRICULTURAL SCIENCE (UNIVERSITY OF TABRIZ), 22(2), 67-78. https://www.sid.ir/en/journal/ViewPaper.aspx?id=276572



Vancouver: Copy

GHASEMZADEH A., SOKHANDANBASHIR N., KHAKVAR R.. MOLECULAR DETECTION OF POTATO VIRUS Y USING UNIVERSAL PRIMERS FROM ARDABIL PROVINCE. JOURNAL OF AGRICULTURAL SCIENCE (UNIVERSITY OF TABRIZ). 2012 [cited 2021August01];22(2):67-78. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=276572



IEEE: Copy

GHASEMZADEH, A., SOKHANDANBASHIR, N., KHAKVAR, R., 2012. MOLECULAR DETECTION OF POTATO VIRUS Y USING UNIVERSAL PRIMERS FROM ARDABIL PROVINCE. JOURNAL OF AGRICULTURAL SCIENCE (UNIVERSITY OF TABRIZ), [online] 22(2), pp.67-78. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=276572.



 
 
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