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Paper Information

Journal:   MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY   FALL 2012 , Volume 22 , Number 3 (69); Page(s) 184 To 190.
 
Paper: 

CLONINGVIRG GENE AND GENERATION MUTANT CONSTRUCT OF PGEM VIRG IN ORDER TO INDUCTION RECOMBINATION IN NATIVE SHIGELLA DYSENTERIAE

 
 
Author(s):  AHMADI DANESH HORA, SAADATI MOJTABA*, DOROUDIAN MOHAMMAD, YAKHCHALI BAGHER, KARKHANEH ALI ASGHAR, HOSSEINI SAYED MOSTAFA, ZARE MOKHTAR, HASHEMI MEHRAD
 
* DEPARTMENT OF CELL BIOLOGY, FACULTY OF SCIENCE, IMAM HOSSEIN UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background: Shigellosis disease is the major causes of morbidity in children with diarrhea in Iran.
ThevirG (icsA) gene plays a key role in pathogenesis and ability of invasion in shigella. The aim of this study was cloning, sequencingvirG gene and developing a mutant construct pGEM
D virG in order to induction recombination in a native shigella for generation a live attenuated vaccine candidate strain.
Materials and methods: Initially, by use of biochemical tests, the native shigella strain was detected.
ThevirG gene was cloned in pGEM-7zf vector and the nucleotide sequence was determined.
According to the data of sequencing, digestion mapping of pGEMvirG vactor was obtained and a part ofvirG gene by using enzymatic digestion was removed. Finally, pGEM
D virG construct was transformed toE. coli by utilization of chemical transformation method.
Results: The native shigella strain by using biochemical tests was confirmed. The result of sequencing virG gene (native strain) was submitted in NCBI Genebank database. The pGEM
D virG construct contains a mutant construct ofvirG gene which 1751 bp was deleted through enzymatic digestion reaction and transformed inE. coli.
Conclusion: Using the technique of allelic exchange based on the incident of recombination in bacteria is one of the most effective methods to develop a disruption in the target genes. This mutant construct can be applied in development of a live attenuatedShigella dysenteriae vaccine candidate.

 
Keyword(s): ALLELIC EXCHANGE, SHIGELLA DYSENTERIAE, VIRG GENE, LIVE ATTENUATED VACCINE CANDIDATE
 
 
References: 
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Citations: 
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APA: Copy

AHMADI DANESH, H., & SAADATI, M., & DOROUDIAN, M., & YAKHCHALI, B., & KARKHANEH, A., & HOSSEINI, S., & ZARE, M., & HASHEMI, M. (2012). CLONINGVIRG GENE AND GENERATION MUTANT CONSTRUCT OF PGEM VIRG IN ORDER TO INDUCTION RECOMBINATION IN NATIVE SHIGELLA DYSENTERIAE. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 22(3 (69)), 184-190. https://www.sid.ir/en/journal/ViewPaper.aspx?id=275913



Vancouver: Copy

AHMADI DANESH HORA, SAADATI MOJTABA, DOROUDIAN MOHAMMAD, YAKHCHALI BAGHER, KARKHANEH ALI ASGHAR, HOSSEINI SAYED MOSTAFA, ZARE MOKHTAR, HASHEMI MEHRAD. CLONINGVIRG GENE AND GENERATION MUTANT CONSTRUCT OF PGEM VIRG IN ORDER TO INDUCTION RECOMBINATION IN NATIVE SHIGELLA DYSENTERIAE. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY. 2012 [cited 2021June18];22(3 (69)):184-190. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=275913



IEEE: Copy

AHMADI DANESH, H., SAADATI, M., DOROUDIAN, M., YAKHCHALI, B., KARKHANEH, A., HOSSEINI, S., ZARE, M., HASHEMI, M., 2012. CLONINGVIRG GENE AND GENERATION MUTANT CONSTRUCT OF PGEM VIRG IN ORDER TO INDUCTION RECOMBINATION IN NATIVE SHIGELLA DYSENTERIAE. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, [online] 22(3 (69)), pp.184-190. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=275913.



 
 
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