Paper Information

Journal:   MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY   FALL 2012 , Volume 22 , Number 3 (69); Page(s) 175 To 183.
 
Paper: 

GROWTH INHIBITORY AND DIFFERENTIATING EFFECTS OF ETHYL ACETATE SOLUBLE METABOLITE OF IRANIAN NATIVE BACTERIA, STREPTOMYCES CALVUS, IN HUMAN MYELOID LEUKEMIA K562 CELL LINE

 
 
Author(s):  MOUSAVI SEYED MOHAMMAD AMIN*, GHANBARVAND FARIDEH, DEHNAD ALI REZA
 
* DEPARTMENT OF ZOOLOGY, FACULTY OF NATURAL SCIENCE, UNIVERSITY OF TABRIZ, TABRIZ, IRAN
 
Abstract: 

Background: Because drug resistance is one of the most important problems in patients with chronic myeloid leukemia (CML), finding new anti-cancer drugs especially from natural sources is research priority. Therefore, in this study, anti-cancer effects of ethyl acetate soluble metabolite of Iranian native bacteria, Streptomyces calvus, were studied using human chronic myeloid leukemia K562 cells.
Materials and methods: In this experimental trail, ethyl acetate soluble metabolites were isolated fromS. calvus bacteria. K562 cell line was treated by various concentrations of this metabolite for 12-72h intervals. Anti-proliferative effects of ethyl acetate soluble metabolite were studied by trypan blue exclusion test. Wright-Giemsa staining and latex particle phagocytosis assay were used to study differentiated cells.
Results: Ethyl acetate soluble metabolite induced growth inhibition in K562 cells in concentration and time- dependent manner. At the concentration of 200 ng/ml, the growth was inhibited 19-50% after 12-72h. Latex particle phagocytosis assay and Wright- Giemsa staining results revealed that K562 cells were differentiated toward monocytic- macrophagic lineage.
Conclusion: According to growth inhibitory and differentiating effects of S.calvus metabolite in K562 cells, this metabolite can be proposed for more investigations in differentiation therapy of CML patients.

 
Keyword(s): CHRONIC MYELOID LEUKEMIA, DIFFERENTIATION, NATURAL PRODUCTS, STREPTOMYCES
 
References: 
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