Paper Information

Journal:   JOURNAL OF CELL & TISSUE   SPRING 2012 , Volume 3 , Number 1; Page(s) 73 To 81.
 
Paper: 

RESEARCH ARTICLE: HISTOMORPHOLOGY AND HISTOMETRIC STUDY OF THE HEAD’S SKIN IN (BARBUS GRYPUS)

 
Author(s):  BASIR Z., MOROVVATI H.*, KHAKSARI M., MESBAH M., ABDI R.
 
* DEPARTMENT OF BASIC SCIENCE, FACULTY OF VETERINARY MEDICINE, AHVAZ UNIVERSITY OF SHAHID CHAMRAN
 
Abstract: 

Aim: As there was no study available regarding the morphological structure of skin of (Barbus grypus) therefore this research work was performed to help the other studies such as histopathology of the fish.
Material and Methods: Skin of the different part of the head of 20 Barbus grypus with 5mm thickness were removed and fixed in bouin solution. The routine procedures for preparation of tissues were followed. For transmission electron microscopic study, the samples after primary and post-fixation were dehydrated and embedded in resin. Then, thin sections 50 µm were prepared and stained with uranyl acetate.
Results: according to histomorphological examination, skin of head, lip and barbel was made of epidermis and dermis which were located on a loose connective tissue called hypoderm. In histometric study, skin of studied area, was divided into different regions in (Barbus grypus) based on cells distribution. In the micrograph of TEM, goblet cells containing many mucus granule, spinous and club cells with hyper chromatin nucleus were observed, which presence of hyper chromatin nucleus was more obvious in the club cells?
Conclusion: The light and TEM studies on the skin in studied region of (Barbus grypus), showed there was differences and some similarity with the other reported species.

 
Keyword(s): BARBUS GRYPUS, HISTOMORPHOLOGY, HISTOMETRIC, SKIN
 
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