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Paper Information

Journal:   JOURNAL OF CELL & TISSUE   SPRING 2012 , Volume 3 , Number 1; Page(s) 21 To 27.
 
Paper: 

RESEARCH ARTICLE: THE STAGES OF SOMATIC EMBRYOGENESIS DERIVED FROM ZYGOTIC EMBRYO OF DOREMA AMMONIACUM D.

 
 
Author(s):  GHASEMIAN KH., NAZERI S.*, CHEHREGANI RAD A., MIRZAIE ASL A.
 
* HAMEDAN, BU-ALI-SINA UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF BIOTECHNOLOGY
 
Abstract: 

Aim: The aim was to study the histology of somatic embryogenesis stages in Dorema ammoniacum D. using tissue cultures as an efficient method for propagation of the plants with restriction in cultivation.
Material and methods: Various explants were cultured on MS (Murashige and Skoog) medium containing 30 g l-1 sucrose and 7 g l-1 agar as well as supplemented with different concentrations of auxin and cytokinin. After eight weeks, different stages of development of somatic embryos in embryogenic callus were evaluated using histological method.
Results: Out of different explants, only zygotic embryo was able to induce embryogenic callus. Presence of NAA (
a-Naphthaleneacetic acid) increased callus induction and somatic embryogenesis. The highest callus formation was observed in MS medium supplemented with 1 mg/L NAA and 0.5 mg/L BA (N6-Benzyladenine). Different stages of somatic embryos including: globular, heart-shaped, torpedo and cotyledonus stages were observed in the prepared sections of callus.
Conclusion: Using zygotic embryo the in vitro somatic embryogenesis of Dorema ammoniacum D. was possible. In addition the histological examinations of embryogenic callus showed the existence of the different stages of embryonic development.

 
Keyword(s): DOREMA AMMONIACUM, HISTOLOGY, SOMATIC EMBRYOGENESIS
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

GHASEMIAN, K., & NAZERI, S., & CHEHREGANI RAD, A., & MIRZAIE ASL, A. (2012). RESEARCH ARTICLE: THE STAGES OF SOMATIC EMBRYOGENESIS DERIVED FROM ZYGOTIC EMBRYO OF DOREMA AMMONIACUM D.. JOURNAL OF CELL & TISSUE, 3(1), 21-27. https://www.sid.ir/en/journal/ViewPaper.aspx?id=275844



Vancouver: Copy

GHASEMIAN KH., NAZERI S., CHEHREGANI RAD A., MIRZAIE ASL A.. RESEARCH ARTICLE: THE STAGES OF SOMATIC EMBRYOGENESIS DERIVED FROM ZYGOTIC EMBRYO OF DOREMA AMMONIACUM D.. JOURNAL OF CELL & TISSUE. 2012 [cited 2021July30];3(1):21-27. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=275844



IEEE: Copy

GHASEMIAN, K., NAZERI, S., CHEHREGANI RAD, A., MIRZAIE ASL, A., 2012. RESEARCH ARTICLE: THE STAGES OF SOMATIC EMBRYOGENESIS DERIVED FROM ZYGOTIC EMBRYO OF DOREMA AMMONIACUM D.. JOURNAL OF CELL & TISSUE, [online] 3(1), pp.21-27. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=275844.



 
 
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