Paper Information

Journal:   MODARES JOURNAL OF MEDICAL SCIENCES (PATHOBIOLOGY)   SUMMER 2010 , Volume 13 , Number 2; Page(s) 73 To 78.
 
Paper: 

SHORT COMMUNICATION: THE STUDY OF IGG IMMUNOGLOBOLIN ANTI CRYPTOSPORIDUM PARVUM IN INFECTED NEONATAL BALB/C MICE

 
 
Author(s):  AHMADIAN MASOUMEH, SADRAEI JAVID*, ZAVARAN HOSSEINI AHMAD
 
* DEPARTMENT OF PARASITOLOGY AND ENTOMOLOGY, FACULTY OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN, P.O.CODE: 1411713116
 
Abstract: 

Objective: Cryptosporidiosis is a parasitic disease that is causing small protozoan genus Cryptosporidum and transmission take place through fecal- oral by direct contact or indirectly through food or drink. The aim of this study was detection of anti-Cryptosporidum parvum Immunoglobulin IgG, in newborn BALB/c infected with C. parvum.
Materials and Methods: Oocysts of C. parvum were obtained from the feces of diarrheic lambs and following purification they were suspended in 2.5% aqueous potassium dichromate solution and stored at 4oC. Forty suckling BALB/c (3-4 days old) were divided in 8 groups (4 case groups and 4 control groups) each group consist of 5 suckling BALB/c. The mice in case groups were infected oraly with 105 C. parvum oocysts, and the mice in control groups served as non-infected. Blood samples were collected at 6, 9, 12 and 16 days post-infection (pi). Immunoglobulin IgG were extracted by salting out method and confirmed with SDS-PAGE.
Results: Antibodies were analyzed by western blot and increased secretion of IgG was confirmed in neonatal mice infected with Cryptosporidum oocysts. Mean OD of Immunoglobulin IgG increased from 0.350
±0.099 to 0.6776±0.099 in case groups but in control groups the increase was from 0.244±0.016 to 0.322±0.16 (P<0.05).
Conclusion: The type of antibody in neonetal mice infected with Cryptosporidum oocysts was IgG which is secreted against external memberane of oocysts. Significant differences in neonatal mice case groups as compared with the control groups were observed.

 
Keyword(s): CRYPTOSPORIDUM PARVUM, IGG, WESTERN BLAT, BALB/C
 
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