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Paper Information

Journal:   PAJOUHESH-VA-SAZANDEGI   WINTER 2009 , Volume 21 , Number IN AGRONOMY AND HORTICULTURE (SPECIAL ISSUE); Page(s) 139 To 146.
 
Paper: 

IN VITRO INVESTIGATION OF ASPERGILLUS FLAVUS INHIBITION GROWTH AND AFLATOXIN B1 UPTAKE BY LACTOBACILLUS PLANTARUM

 
 
Author(s):  KHANAFARI A.*, SOUDI H., MIRABOULFATHI M., KARAMI OSBOO R.
 
* MICROBIOLOGY DEPARTMENT, ISLAMIC AZAD UNIVERSITY NORTH OF TEHRAN BRANCH, IRAN
 
Abstract: 

Laboratory studies assessed the binding of aflatoxin B1 (AFB1) and its adsorption by many strains of lactic acid bacteria which werw used in foods. In this study the binding ability of AFB1? a main endoded Lactobacillus plantarum (PTCC 1058) was studied. The 0.5 ppm AFB1 solution was inoculated at 37oC for 1, 24, 48, 90 hours and the AFB1 residues were quantitively in supernatant by HPLC. Results showed this strain removed 45% of AFB1 from solution within 1h and 100% of it in 90h. In death phase, this bacteria removed only 31% of AFB1 from solutions after 1 and 24h, but this value reduced to 15% after 48 and 90h incubation periods (P0.05). The stabilities of the amount of AFB1 to bacterium cell wall were evaluated by usign organic solvent (chloroform) and the result indicates no AFB1 extraction by solvent. The low amount aflatoxin production by three different methods represented high level of fungus growth suppression by lactobacillus.

 
Keyword(s): AFB1, LACTOBACILLUS PLANTARUM, HPLC
 
References: 
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