Paper Information

Journal:   IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE   JANUARY 2010 , Volume 14 , Number 47; Page(s) 7 To 11.
 
Paper: 

APOPTOSIS FEATURE IN DERMATOPHYTE FUNGI OF EPIDERMOPHYTON FLOCCOSUM, MICROSPORUM CANIS AND TRICHOPHYTON MENTAGROPHYTES

 
Author(s):  BEHZADI P.*, BEHZADI E., GERAMI SHOAR M.
 
* 
 
Abstract: 
Background and Objective: Until yet, there is no investigation in the field of apoptosis feature in UV-light exposed dermotophyte fungi of Epidennophyton floccosum, Microsporum canis and Trichophyton mentagrophytes. So, in this study, we tried to detect the Apoptosis feature in dermatophyte fungi of Epidermophyton floccosum, Microsporum canis and Trichophyton mentagrophytes. Then the results were analyzed by molecular methods.
Materials and methods: The colonies of mentioned dermatophytes were irradiated by UV light at the wavelength of 302 nm. To investigate the possible Apoptosis feature, the high molecular-weight DNA was isolated from non-irradiated and irradiated colonies of dermatophytes respectively and then were run through 1% agarose gel.
Results: Isolated DNA molecules, showed no differences between DNA banding patterns. By the performed protocol in this project, no feature of Apoptosis was detected in the dermatophyte fungi of Epidermophyton floccosum, Microsporum canis and Trichophyton mentagrophytes.
Conclusions: By the help of this UV-irradiation protocol, it is impossible to obeserve the apoptosis feature in dermatophyte fungi of Epidermophyton floccosum, Microsporum canis and Trichophyton mentagrophytes. Maybe the negative tropism of these fungi against UVB is the main reason of no Apoptosis feature.
 
Keyword(s): APOPTOSIS, UV LIGHT, EPIDERMOPHYTON FLOCCOSUM, MICROSPORUM CANIS, TRICHOPHYTON MENTAGROPHYTES
 
References: 
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