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Paper Information

Journal:   IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE   JULY 2010 , Volume 15 , Number 49; Page(s) 55 To 61.
 
Paper: 

IPAB GENE IN SHIGELLA

 
 
Author(s):  EGHTEDARDOOST M., SAADATI M.*, NAZARIAN SH., ZARE MOKHTAR, MALALI F., HEIAT M.
 
* 
 
Abstract: 
Background and objective: Shigelosis is one of five important infectious diseases. Infection by different species of Shigella (gram-negative bacterium) results in dysentery, an acute inflammatory disease of the colon. Many attempts carried out for produce an efficient vaccine aginst shigella and there are more hope to immunogenic elements of shigella such as invasive plasmid antigen (IpaB, IpaC).
Materials and methods: ipaB gene was extracted and amplificated as a template. Then the PCR product was cloned into pGEM-T vector and then subclone into PET22b (+). Finally plasmid was extracted from transformed Escherichia coli (TOPIO strain) and subclon confirmed by enzyme digestion as well as, nested PCR.
Results: ipaB gene detected in all strains of shigella; PCR and nested PCR and enzyme digestion confirmed ipaB gene cloned into PET22b (+).
Conclusion: According to detection of ipaB gene in all shigella strain, we could use these primers in clinical diagnosis. We cloned ipaB gene of S. dysenteriae successfully. Recombinant plasmid was confirmed. It prepare for future studies such as expression of this protein and assay of immunogenicity, then present this recombinant plasmid as a candid for recombinant vaccine of shigellosis.
 
Keyword(s): IPAB GENE, SHIGELLA DYSENTERIAE, PCR, ENZYME DIGESTION
 
References: 
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