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Paper Information

Journal:   IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE   JULY 2010 , Volume 15 , Number 49; Page(s) 49 To 53.
 
Paper: 

DETECTION OF TOXICOGENIC E.COLI 0157:H7 BY PCR

 
 
Author(s):  TAVAKOLI H.R., GHORBAN ALIZADEGAN M., NAJAFI A.*, AHMADI A.
 
* 
 
Abstract: 
Background and objective: E. coli Isolation rate by culture-method will be reduced if animals take antibiotics before sampling. This study aimed to setup, optimize and introduce a sensitive and specific PCR detection method for identification of E. coli DNA in clinical samples.
Materials and methods: stx2A gene was selected as a specific target sequence. This primer pair amplifies 556 bp of this target gene. E. coli 0157H7 was used as a standard organism for optimization experiments. Phenol – Chloroform method was used for DNA extraction. Amplified product was detected by 1% gel agarose electrophoresis, stained by ethidiome bromide.
Results: Provided data confirmed amplification of expected product. Specificity test proved no cross reaction with tested organisms. Sensitivity test detected 500fg E.coli DNA as a final detection limit.
Conclusion: Optimized experiment confirming applied PCR protocols is quite fast with high sensitivity and specificity performing in less than three hours.
Application of this test to the clinical laboratories can help to rapid diagnosis of the E.coli in samples.
 
Keyword(s): E.COLI, PCR, STX2A GENE
 
 
References: 
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Citations: 
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APA: Copy

TAVAKOLI, H., & GHORBAN ALIZADEGAN, M., & NAJAFI, A., & AHMADI, A. (2010). DETECTION OF TOXICOGENIC E.COLI 0157:H7 BY PCR. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 15(49), 49-53. https://www.sid.ir/en/journal/ViewPaper.aspx?id=274842



Vancouver: Copy

TAVAKOLI H.R., GHORBAN ALIZADEGAN M., NAJAFI A., AHMADI A.. DETECTION OF TOXICOGENIC E.COLI 0157:H7 BY PCR. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE. 2010 [cited 2021July27];15(49):49-53. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274842



IEEE: Copy

TAVAKOLI, H., GHORBAN ALIZADEGAN, M., NAJAFI, A., AHMADI, A., 2010. DETECTION OF TOXICOGENIC E.COLI 0157:H7 BY PCR. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, [online] 15(49), pp.49-53. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274842.



 
 
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