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Paper Information

Journal:   IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE   JULY 2010 , Volume 15 , Number 49; Page(s) 17 To 22.
 
Paper: 

MECA GENE AMONG HIGH LEVEL METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM TEHRAN HOSPITALS

 
 
Author(s):  JAVAN A., FALAHATI H.R., SAIFI M.*, TALEBI M., EBRAHIMPOUR GH., POURSHAFI M.R.
 
* 
 
Abstract: 
Background and objective: Due to the major diffusion of methicillin resistant Staphylococcus aureus (MRSA) strains in hospital settings, prevention and control strategies are receiving increased attention. Phenotypic methods for detection of MRSA have been compared with the gold standard which, as of now, is by the detection of mecA gene by polymerase chain reaction (PCR).
Material and methods: The study was conducted from October 2008 to August 2009 and included 350 staphylococci isolates. All Staphylococcus aureus isolates were tested for methicillin resistance using oxacillin disk diffusion and MIC and presence of mecA gene by PCR. Antibiotic resistance of MRSA isolates were accomplished according to the CLSI guidelines.
Results: Out of 350 samples 150 Staphylococcus aureus isolates were obtained and MRSA was identified in 68 (45%) samples. All of the MRSA isolates were resistant to penicillin and amikacin and resistance percents among MRSA isolates to kanamycin, ciprofloxacin, tobramycin, erythromycin, gentamicin, tetracycline, clindamycin and co-trimoxazole were higher than 80%. The MIC results for 25 (37%) and 40 (59%) of MRSA isolates were
³512 mg/ml and ³256 mg/ml respectively. The PCR method indicated the presence of mecA gene in 64 (95%) of MRSA isolates.
Conclusion: Prevalence of high level methicillin resistance among MRSA isolates is quite high and co-resistance to several antibiotics was detected during the present study. Active surveillance should be considered for the prevention of an outbreak of this type of MRSA in hospital settings.
PCR facilitates the rapid detection of MRSA and has the potential to contribute to preventing spread, but should continue to be used in conjunction with culture and drug resistance tests.
 
Keyword(s): MECA, METHICILLIN, STAPHYLOCOCCUS AUREUS
 
References: 
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