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Paper Information

Journal:   IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE   JULY 2010 , Volume 15 , Number 49; Page(s) 1 To 5.
 
Paper: 

HIGH FREQUENCY OF MIXED GENOTYPES ROTAVIRUS AMONG CHILDREN HOSPITALIZED WITH ACUTE GASTROENTERITIS. 2007-2008

 
 
Author(s):  KARGAR M.*, ZARE M.
 
* 
 
Abstract: 
Background and objective: Rotaviruses are the most important cause of severe viral gastroenteritis and dehydration diarrhea in children under five years old worldwide. This study conducted to determine genotypes of protein 7 human rotaviruses with Nested RT- PCR among children hospitalized in Marvdasht During 2007 to 2008.
Material and methods: In this study, 141 stool samples of under 5 years old hospitalized children with severe diarrhea were collected from Motahary hospital in Marvdasht. At first group A Rotaviruses were distinguished by Enzyme Immunoassay (EIA) and then, the genotypes of positive samples were determined by using 9 specific primers by Nested RT-PCR method.
Results: Out of total collected samples, 40 were positive by EIA. The frequency of G1, G2 and G4 Genotypes were 5.0%, 12.5% and 10.0% respectively. G3, G8 and G9 were detected in none of specimens. The frequency of mixed and non-typeable genotypes was determind 60% and "12.5% respectively.
Conclusions: Frequency of mixed genotypes indicates severe infection because of low sanitation or transmission of recombinant strains from animal to humans. Also, the noticeable frequency of non-typeable genotypes indicates the necessity of using other primers for identification of unusual genotypes.
 
Keyword(s): HUMAN ROTAVIRUSES, GENOTYPING, MIXED GENOTYPES, NESTED RT- PCR
 
 
References: 
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Citations: 
 
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APA: Copy

KARGAR, M., & ZARE, M. (2010). HIGH FREQUENCY OF MIXED GENOTYPES ROTAVIRUS AMONG CHILDREN HOSPITALIZED WITH ACUTE GASTROENTERITIS. 2007-2008. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 15(49), 1-5. https://www.sid.ir/en/journal/ViewPaper.aspx?id=274793



Vancouver: Copy

KARGAR M., ZARE M.. HIGH FREQUENCY OF MIXED GENOTYPES ROTAVIRUS AMONG CHILDREN HOSPITALIZED WITH ACUTE GASTROENTERITIS. 2007-2008. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE. 2010 [cited 2021July24];15(49):1-5. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274793



IEEE: Copy

KARGAR, M., ZARE, M., 2010. HIGH FREQUENCY OF MIXED GENOTYPES ROTAVIRUS AMONG CHILDREN HOSPITALIZED WITH ACUTE GASTROENTERITIS. 2007-2008. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, [online] 15(49), pp.1-5. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274793.



 
 
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