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Paper Information

Journal:   JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)   WINTER 2010 , Volume 19 , Number 74/1 (BIOLOGY ISSUE); Page(s) 107 To 124.
 
Paper: 

HAKIMEH OIL FIELD BY EVALUATING THE ROLE OF BACILLUS IN PRODUCING BIOSURFACTANTS AND THE FEASIBILITY OF MICROBIOLOGIC

 
 
Author(s):  AKHAVAN SEPAHI A.*, MOSTAFAEE R.
 
* MICROBIOLOGY DEPARTMENT, NORTH TEHRAN BRANCH, ISLAMIC AZAD UNIVERSITY, TEHRAN, IRAN
 
Abstract: 
Introduction: We recently witnessed that due to the aging of the oil wells there is a significant pressure drop in the oil reservoirs. In order to case study on Bibi Hakimeh oil filed to consider, the structural conditions, reservoir parameters, geological location, biological characteristics and etc.
Aim: To evaluate and start the selection of the best strain, optimum growing conditions and Bio surfactants production by Ex-Situ method in the Asmari reservoir.
Materials and Methods: A high temperature resistant Bacillus strain has been selected for evaluating the production ability of Bio surfactants. It has been selected after performing all morphological biochemical and genetic studies; the temperature, PH, Carbon, N2, resistance against the temperature and other factors have been optimized for Bio surfactants production.
Result: In north mean part of the field studied, thickness average of Asmari formation is about 450 meters and average temperature in Asmari source than other fields with respect to the building conditions. Indicates significant reduction (of temperature or oil production).
As the result of the results from morphological methods, biochemical and genetically tests done we can conclude that our bacteria of interest is Bacillus licheniformis. The minimum surface tension values obtained for strain cultivation environments for Bacillus licheniformis separated 30mN/m. compared with values reported for other bio-surfactant reproductive bacteria, these results are in acceptable limits.
Conclusion: (one of the best carbon sources is crude oil. PH effectiveness on producing bio-surfactant from this type of bacteria indicated that it can produce bio-surfactant at a pH 6 to 8 but best pH is between 6.5 to 7.5. We also found that emulsion activation for our best strain was 91o SBB5. Nitrogen is one of the best sources for growing and producing bio-surfactant: strain can reduce surface tension in 30mN/m. meanwhile, sucrose and glucose reduce this tension to 30mN/m. The results of reservoir pressure reduction, structural and petro physical condition II) oil fields have been assessed. According to PVT characteristics of the reservoir with respect to the drive system it is is a dual system and a few years ago pressure dropped in both systems, water and gas drive but gas drive is more rigorous. Then supplying reservoir with pressure and preventing its pressure reduction can playa significant role in increasing lifetime of the reservoir .Temperature in Asmari reservoir ranges between 65 to 120 o and it is possible to grow Bacillus with respect to the reservoir conditions. Asmari Formation has various breakings formations, porosity and water saturation in various wells in formation studied is 7 percent and less than 50 percent.
 
Keyword(s): BIBI HAKIMEH OILFIELD, ASMARI RESERVOIR AND OIL RECOVERY
 
 
References: 
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Click to Cite.
APA: Copy

AKHAVAN SEPAHI, A., & MOSTAFAEE, R. (2010). HAKIMEH OIL FIELD BY EVALUATING THE ROLE OF BACILLUS IN PRODUCING BIOSURFACTANTS AND THE FEASIBILITY OF MICROBIOLOGIC. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), 19(74/1 (BIOLOGY ISSUE)), 107-124. https://www.sid.ir/en/journal/ViewPaper.aspx?id=274491



Vancouver: Copy

AKHAVAN SEPAHI A., MOSTAFAEE R.. HAKIMEH OIL FIELD BY EVALUATING THE ROLE OF BACILLUS IN PRODUCING BIOSURFACTANTS AND THE FEASIBILITY OF MICROBIOLOGIC. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY). 2010 [cited 2021May07];19(74/1 (BIOLOGY ISSUE)):107-124. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274491



IEEE: Copy

AKHAVAN SEPAHI, A., MOSTAFAEE, R., 2010. HAKIMEH OIL FIELD BY EVALUATING THE ROLE OF BACILLUS IN PRODUCING BIOSURFACTANTS AND THE FEASIBILITY OF MICROBIOLOGIC. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), [online] 19(74/1 (BIOLOGY ISSUE)), pp.107-124. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274491.



 
 
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