Paper Information

Journal:   JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES   SUMMER 2012 , Volume 20 , Number 2; Page(s) 38 To 43.
 
Paper: 

STUDY OF MUTATION FREQUENCY IN PA3721 GENE AMONG PRODUCING AND NON-PRODUCING ESBLS PSEUDOMONAS AERUGINOSA STRAINS BY PCR METHOD

 
 
Author(s):  VALIZADEH A., SADEGHIFARD N.*, ZOLFAGHARY M.R., MALEKI A., GHAFOURIAN S., SHAKIB P.
 
* CLINICAL MICROBIOLOGY RESEARCH CENTER, ILAM UNIVERSITY OF MEDICAL SCIENCES, ILAM, IRAN
 
Abstract: 

Introduction: Some Pseudomonas aeruginosa strains produce Extended Spectrum Beta Lactamase which causes resistance to cephalosporins such as cefteriaxon, cefotaxim, ceftazidim and also monobactams such as azteronam. Over expression of efflux pumps including MexAB-OPRM is a factors which play an important role in resistance of Pseudomonas aeruginosa strains to antibiotics.
Materials & Methods: In order to detect the mutation frequency in PA3721 gene, 50 isolates of Pseudomonas aeruginosa isolated from urinary tract infection in Emam Khomeini Hospital of Ilam and Milad Hospitalin Tehran were studied. The isolates were confirmed by biochemical methods and the Extended Spectrum Beta Lactamase were screened by disk diffusion method. Phenotypic confirmatory test by combined disk was utilized for confirmation of ESBLs. In next step, ESBLs genes as well as mutation in frequency PA3721 gene in producing and non producing ESBLs Pseudomonas aeruginosa strains were identified by PCR method.
Findings: In this study, 50 isolates were studied, including 18 isolates from Milad Hospital and the rest from Ilam Hospitals.
In Milad Hospital, ESBLs genes for PER, PSE and VEB and in Ilam Hospitals ESBL gene for OxA-10 were detected. In Ilam samples including 32 isolates of which 17 samples produced ESBLs, all carried oxA- 10 gene and 8 isolates (47.05%) were positive for mutation in PA3721 gene.
Discussion & Conclusion: According to the results, Meropenem was more effective against Pseudomonas aeruginosa isolates in in vitro condition. The results also showed that nearly half of oxA-10 producing Pseudomonas aeruginosa isolates carrynalC gene.

 
Keyword(s): ESBLS, MEX AB-OPRM, PSEUDOMONAS AERUGINOSA
 
References: 
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