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Paper Information

Journal:   AGRICULTURAL SCIENCES AND TECHNOLOGY   2007 , Volume 21 , Number 2 (SPECIAL ISSUE IN HORTICULTURE SCIENCES); Page(s) 79 To 90.
 
Paper: 

STUDY ON THE ROOT ADVENTITIOUS ROOT FORMATION IN SOME HORTICULTURAL CROPS USING AGROBACTERIUM RHIZOGENESE

 
 
Author(s):  AZIZI M.*, AGHABOZORGEE M., FARSEE M., TEHRANIFAR A., ZOLALLA J., GHABOOLIL M.
 
* DEPARTMENT OF HORTICUTURE, GOLLEGE OF AGRICULTURE FERDOWSI UNIVERSITY, MASHHAD, IRAN
 
Abstract: 
One of the main obstacles in vegetative propagation of plants is hard root formation. A. rhizogenes mediated transformation dissolved the problem and could be used as an alternative root induction method. In this research, we used two strains of A. rhizogenes (A4 and GMI9534) for root induction and transformed root production of three stem type (current season growth, 1-2 years old and 5-6 years old stem) in some horticultural crops. This study was conducted in two factorial experiments in randomized complete block design. In first experiment (in vitro) we inoculated shoots of Berberis vulgar var. asperma and sterile seedling of Lavandula spica. In second experiment (In vivo) we inoculated stem cutting (Berberis vulgar var. asperma, Ficus benjamine) and air layered stem of Berberis vulgar var. asperma, Ficus benjamine, Rosa spp, Rosmarinus officinal is with A. rhizogenes for root induction. Results showed that GMI9534 increased root induction of sterile seedling of Lavandula spica significantly (p<0.01) in comparison with A4 and control. Hardwood stem (1-2 years old) of Rosmarinus officinal is that inoculated with GMI9534 significantly (p<0.05) produced more root than A4 and control treatment. Root induction of inoculated stem cutting and layerage (1- 2 years old) in Ficus benjamine and Rosa spp with GMI9534 was significantly (p<0.01) higher than A4 and control. PCR analysis of DNA extracted from transformed root, confirmed that rolC gene has been transferred to adventitious roots by A. rhizogenes.
 
Keyword(s): AGROBACTERIUM RHIZOGENES, A4 AND GMI9534, ADVENTITIOUS ROOT, BERBERIS VULGAR VAR. ASPERN1A, LAVANDULA SPICA, FICUS BENJAMINE AND ROSA SPP.
 
 
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Click to Cite.
APA: Copy

AZIZI, M., & AGHABOZORGEE, M., & FARSEE, M., & TEHRANIFAR, A., & ZOLALLA, J., & GHABOOLIL, M. (2007). STUDY ON THE ROOT ADVENTITIOUS ROOT FORMATION IN SOME HORTICULTURAL CROPS USING AGROBACTERIUM RHIZOGENESE. AGRICULTURAL SCIENCES AND TECHNOLOGY, 21(2 (SPECIAL ISSUE IN HORTICULTURE SCIENCES)), 79-90. https://www.sid.ir/en/journal/ViewPaper.aspx?id=274337



Vancouver: Copy

AZIZI M., AGHABOZORGEE M., FARSEE M., TEHRANIFAR A., ZOLALLA J., GHABOOLIL M.. STUDY ON THE ROOT ADVENTITIOUS ROOT FORMATION IN SOME HORTICULTURAL CROPS USING AGROBACTERIUM RHIZOGENESE. AGRICULTURAL SCIENCES AND TECHNOLOGY. 2007 [cited 2021May08];21(2 (SPECIAL ISSUE IN HORTICULTURE SCIENCES)):79-90. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274337



IEEE: Copy

AZIZI, M., AGHABOZORGEE, M., FARSEE, M., TEHRANIFAR, A., ZOLALLA, J., GHABOOLIL, M., 2007. STUDY ON THE ROOT ADVENTITIOUS ROOT FORMATION IN SOME HORTICULTURAL CROPS USING AGROBACTERIUM RHIZOGENESE. AGRICULTURAL SCIENCES AND TECHNOLOGY, [online] 21(2 (SPECIAL ISSUE IN HORTICULTURE SCIENCES)), pp.79-90. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274337.



 
 
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