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Paper Information

Journal:   VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)   SPRING 2012 , Volume 25 , Number 1 (94); Page(s) 25 To 30.
 
Paper: 

Comparison Of The Validity Of Cie And Pcr Diagnostic Methods In Determining Hydatidosis In Sheep

 
 
Author(s):  Yakhchali M.*, Morshedi A., Shirashian M.
 
* DEPARTMENT OF PATHOBIOLOGY, PARASITOLOGY DIVISION, FACULTY OF VETERINARY MEDICINE, URMIA UNIVERSITY, URMIA, IRAN
 
Abstract: 
Sheep is one of the important hosts for hydatidosis in Iran as a hyperendemic region worldwide. In the present study, the efficacy of counter immuno-electerophoresis (CIE) and PCR tests to detect sheep hydatid cyst infection was carried out. For this, soluble antigen was provided using hydatid cyst fluids (HCFs). Blood sera were taken from a total number of 100 slaughtered sheep. CIE analysis was undertaken using negative and positive controls. DNA was extracted from protoscolices and a 1213bp fragment length of co1 gene amplified. Necropsy findings indicated that lung and liver of slaughtered sheep (33%) was infected with hydatid cyst. While it was 29% in CIE and 28% in PCR test. According to the necropsy findings as a gold standard test, sensitivity and specificity of CIE was 79.31% and 92.2%, respectively. Sensitivity and specifity of PCR test were determined 85.19% and 94.8%, as well. Data analysis showed that there is no significant difference between these two techniques (P<0.05). Based on these results, CIE technique can be considered as a useful method to screen sheep hydatid cyst infection.
 
Keyword(s): HYDATID CYST, SHEEP, CIE, PCR
 
 
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APA: Copy

YAKHCHALI, M., & MORSHEDI, A., & SHIRASHIAN, M. (2012). COMPARISON OF THE VALIDITY OF CIE AND PCR DIAGNOSTIC METHODS IN DETERMINING HYDATIDOSIS IN SHEEP. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 25(1 (94)), 25-30. https://www.sid.ir/en/journal/ViewPaper.aspx?id=274188



Vancouver: Copy

YAKHCHALI M., MORSHEDI A., SHIRASHIAN M.. COMPARISON OF THE VALIDITY OF CIE AND PCR DIAGNOSTIC METHODS IN DETERMINING HYDATIDOSIS IN SHEEP. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI). 2012 [cited 2022January24];25(1 (94)):25-30. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274188



IEEE: Copy

YAKHCHALI, M., MORSHEDI, A., SHIRASHIAN, M., 2012. COMPARISON OF THE VALIDITY OF CIE AND PCR DIAGNOSTIC METHODS IN DETERMINING HYDATIDOSIS IN SHEEP. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), [online] 25(1 (94)), pp.25-30. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274188.



 
 
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