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Paper Information

Journal:   THE SCIENTIFIC JOURNAL OF AGRICULTURE (SJA)   MARCH 2008 , Volume 30 , Number 4-A; Page(s) 1 To 8.
 
Paper: 

THE EFFECTS OF DIFFERENT CONCENTRATION OF SOME CHEMICALS ON IN VITRO POLLEN GRAIN GERMINATION OF THREE KHUZESTAN MALE DATE CULTIVARS

 
 
Author(s):  MORTAZAVI S.M.H.*, ARZANI K., MOEINI A.
 
* HORTICULTURAL SCIENCE DEPARTMENT, SHAHID CHAMRAN UNIVERSITY OF AHVAZ, IRAN
 
Abstract: 

Date palm (Phoenix dactylifera L.) is a dioecious plant in which artificial pollination is an essential cultural practice to obtain satisfactory fruit set. Use of viable and high quality pollen grains is the most important factor in fruit production and breeding programs. This research was conducted to study the in vitro pollen germination of three male date cultivars under varying concentrations of sucrose, boric acid and calcium nitrate. The experiment was implemented as a factorial in completely randomized designs with three replications. Experimental factors were three male date cultivars. (Ghanami, Samsmavi and Gheibani), four concentrations of boric acid (0, 50, 100 and 200 mg/Lit), three concentrations of calcium nitrate (0, 200 and 300 mg/Lit) and two sucrose concentration (15% and 25%) in modified Brebaker and Kwack medium. Also a comparison was made between two methods of viability test: in vitro germination and staining with acetocarmine. The results showed that in vitro germination is more effective and precise method for viability test of male date pollens and maximum germination was seen under 50 mg/Lit boric acid, 200 mg/Lit calcium nitrate and 15% sucrose concentration in the medium. Also Samsmavi and Ghanami cultivars had the maximum and minimum viability with 81.8% and 58.4% pollen germination, respectively.

 
Keyword(s): DATE PALM (PHOENIX DACTYLIFERA L.), POLLEN, VIABILITY, IN VITRO GERMINATION, STAINING, MALE CULTIVARS, KHUZESTAN
 
References: 
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