Teucrium polium belongs to Labiatae family that grown wild in most of regions of Iran. Germander (Teucrium polium) is an edible plant which is also widely used in folk medicine. This plant was collected from Broujerd Mountains of Lorestan province in Iran. The air-dried epigeous parts were subjected to hydrodistillation method (in a yield 0.1% w/w). Identification of essential oil costitiuents was made by GC and GC/MS. The antibacterial tests were carried out by drilling well and measuring diameter of inhibition zone. The major component is a-pinene (13.95%), b- caryophyllene (12.35%), germacrene- B (11.74%), b- pinene (8.75%) and limonene (7.60%). The results of the antibacterial activity showed that the oil has notable antibacterial activities aginest of most the Gram- positive and Gram-negative bacteria which these antibacterial activities were more than gentamicine.