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Paper Information

Journal:   PROGRESS IN BIOLOGICAL SCIENCES   SUMMER-FALL 2011 , Volume 1 , Number 2; Page(s) 1 To 10.
 
Paper: 

EVIDENCE FOR AN ASSOCIATION BETWEEN WNT-INDEPENDENT β-CATENIN INTRACELLULAR LOCALIZATION AND OVARIAN APOPTOTIC EVENTS IN NORMAL AND PCO-INDUCED RAT OVARY

 
 
Author(s):  HOSSEIN GHAMARTAJ*, KHANMOHAMMADI MANIJEH, JAROOGHI NEDA, KAZEMNEJAD SOMAYE
 
* DEPARTMENT OF ANIMAL BIOLOGY, SCHOOL OF BIOLOGY, UNIVERSITY COLLEGE OF SCIENCE, UNIVERSITY OF TEHRAN, IRAN
 
Abstract: 

The association of secreted frizzled related protein type 4 (Sfrp4) as an antagonist of Wnt molecules in apoptotic events has been reported previously. Moreover, its increased expression has been reported in the ovary of women with polycystic ovary (PCO). We have demonstrated increased Sfrp4 in PCO-induced rat ovary related to an increased number of apoptotic follicles showing nuclear bcatenin subcellular localization. The aims of present study were twofold 1) to ascertain nuclear bcatenin presence with apoptosis by using immunolocalization of Bax and active cleaved casapase-3, and 2) to elucidate whether Sfrp4 could be an inducer of apoptosis by using isolated rat granulosa cell culture in the presence of recombinant human SFRP4. To this end, immuno- expression of two key molecules in Wnt signaling, GSK3b and b-catenin and apoptotic markers were investigated in normal and PCO-induced rat ovary by daily administration of testosterone propionate (TP) for four weeks. We showed that in PCO-induced as well as in normal ovaries there was nuclear or cytoplasmic subcellular localization of GSK3b and a weak pGSK3bser9 immuno-staining in apoptotic granulosa cells. Interestingly, intracellular b-catenin localization was observed in Bax and active caspase-3 positive granulosa cells in normal as well as in PCO-induced rat ovary. Treatment of granulosa cells with rhSFRP4 showed co-localization of nuclear subcellular b-catenin and active caspase-3 as revealed by double immuno-fluorescence. Our results suggest that rhSFRP4 induces apoptosis and that there is an association between Wnt-independent b- catenin nuclear subcellular localization and apoptotic events of rat ovary.

 
Keyword(s): β-CATENIN, APOPTOSIS, SFRP4, GSK3β, POLYCYSTIC OVARY
 
 
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APA: Copy

HOSSEIN, G., & KHANMOHAMMADI, M., & JAROOGHI, N., & KAZEMNEJAD, S. (2011). EVIDENCE FOR AN ASSOCIATION BETWEEN WNT-INDEPENDENT β-CATENIN INTRACELLULAR LOCALIZATION AND OVARIAN APOPTOTIC EVENTS IN NORMAL AND PCO-INDUCED RAT OVARY. PROGRESS IN BIOLOGICAL SCIENCES, 1(2), 1-10. https://www.sid.ir/en/journal/ViewPaper.aspx?id=274115



Vancouver: Copy

HOSSEIN GHAMARTAJ, KHANMOHAMMADI MANIJEH, JAROOGHI NEDA, KAZEMNEJAD SOMAYE. EVIDENCE FOR AN ASSOCIATION BETWEEN WNT-INDEPENDENT β-CATENIN INTRACELLULAR LOCALIZATION AND OVARIAN APOPTOTIC EVENTS IN NORMAL AND PCO-INDUCED RAT OVARY. PROGRESS IN BIOLOGICAL SCIENCES. 2011 [cited 2021June24];1(2):1-10. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274115



IEEE: Copy

HOSSEIN, G., KHANMOHAMMADI, M., JAROOGHI, N., KAZEMNEJAD, S., 2011. EVIDENCE FOR AN ASSOCIATION BETWEEN WNT-INDEPENDENT β-CATENIN INTRACELLULAR LOCALIZATION AND OVARIAN APOPTOTIC EVENTS IN NORMAL AND PCO-INDUCED RAT OVARY. PROGRESS IN BIOLOGICAL SCIENCES, [online] 1(2), pp.1-10. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=274115.



 
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