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Paper Information

Journal:   AGRICULTURAL SCIENCES AND TECHNOLOGY   2007 , Volume 21 , Number 1 (SPECIAL ISSUE IN PLANT PROTECTION); Page(s) 57 To 65.
 
Paper: 

INCREASING THE DETECTION SENSITIVITY OF RALSTONIA SOLANACEARUM USING THE POST-ENRICHMENT AND NITROCELLULOSE MEMBRANE-ELISA TECHNIQUES

 
 
Author(s):  MOSLEMKHANY K., MOZAFARI J., ALIZADEH A., MOBASSER S.
 
* 
 
Abstract: 
Ralstonia solanacearum, the causal agent of bacterial wilt, is an important pathogen of potato reducing yield in tropical and sub: "tropical regions. Detection of latent infection in seed tuber and soil is essential for production of healthy seeds and control management of the disease. This research was conducted to assess the possibility of using a Nitrocellulose Membran-Enzyme Linked Immuno-Sorbent Assay (NCM-ELISA) technique for detecting latent infection of R. solanacearum in potato seed tubers and soil. Our results showed that the NCM-ELISA technique when followed by a bacterial enrichment stage in the plant and soil extracts could successfully detect 10 CFU/ml of R. solanacearum in infected tuber tissue and 104CFU/ml of these bacteria in the soil extract. Enrichment significantly improved the sensitivity of the test up to one million fold compared to NCM-ELISA without enrichment. Based on these results post-enrichment NCM-ELISA is introduced as a simple, sensitive and rapid diagnostic technique for detection of R. solanacearum in Iran.
 
Keyword(s): BACTERIAL WILT, POTATO, RALSTONIA SOLANACEARUM, NCM-ELISA, ENRICHMENT
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

MOSLEMKHANY, K., & MOZAFARI, J., & ALIZADEH, A., & MOBASSER, S. (2007). INCREASING THE DETECTION SENSITIVITY OF RALSTONIA SOLANACEARUM USING THE POST-ENRICHMENT AND NITROCELLULOSE MEMBRANE-ELISA TECHNIQUES. AGRICULTURAL SCIENCES AND TECHNOLOGY, 21(1 (SPECIAL ISSUE IN PLANT PROTECTION)), 57-65. https://www.sid.ir/en/journal/ViewPaper.aspx?id=273877



Vancouver: Copy

MOSLEMKHANY K., MOZAFARI J., ALIZADEH A., MOBASSER S.. INCREASING THE DETECTION SENSITIVITY OF RALSTONIA SOLANACEARUM USING THE POST-ENRICHMENT AND NITROCELLULOSE MEMBRANE-ELISA TECHNIQUES. AGRICULTURAL SCIENCES AND TECHNOLOGY. 2007 [cited 2021June19];21(1 (SPECIAL ISSUE IN PLANT PROTECTION)):57-65. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=273877



IEEE: Copy

MOSLEMKHANY, K., MOZAFARI, J., ALIZADEH, A., MOBASSER, S., 2007. INCREASING THE DETECTION SENSITIVITY OF RALSTONIA SOLANACEARUM USING THE POST-ENRICHMENT AND NITROCELLULOSE MEMBRANE-ELISA TECHNIQUES. AGRICULTURAL SCIENCES AND TECHNOLOGY, [online] 21(1 (SPECIAL ISSUE IN PLANT PROTECTION)), pp.57-65. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=273877.



 
 
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