Paper Information

Journal:   AGRICULTURAL SCIENCES AND TECHNOLOGY   2007 , Volume 21 , Number 1 (SPECIAL ISSUE IN PLANT PROTECTION); Page(s) 39 To 47.
 
Paper: 

DETECTION OF TYPE A OF BEET NECROTIC YELLOWVEIN VIRUS (BNYVV) USING SPECIFIC PRIMERS IN RTPCR METHOD IN RAZAVI KHORASAN PROVINCE

 
 
Author(s):  GHAROONI KARDANI S., JAFARPOUR B., FALAHATI RASTEGAR M., TABASINEZHAD F.
 
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Abstract: 
Beet necrotic yellow vein virus (BNYVV) is the type member of the genus Benyvirus which causes the disease known as Rhizomania.
From an economic point of view, it is the most important sugar beet virus transmitted by a soil borne fungus, Polymyxa betae Keskin and the vector survives in soil for many years. BNYVV is characterized by rod-shaped particles and has four types known as: A, B, P and J. Types A and B consist only four plus-sense strands of RNA and types P and J contain RNA5 as well. Samples with Rhizomania symptoms were collected from different fields in the main sugar beet growing areas of Razavi Khorasan province in the summer of 2005. Detection of infected samples were Carried out by DAS-ELISA. To do RT-PCR test, total RNA was extracted from roots of the infected samples by using PEG6000 precipitation method and the cDNA was synthesised using random hexamer primers. PCR was performed with specific forward and reverse primers from TGB region on RNA2.
After electrophoresis on 1.5% agarose and 5% polyacrylamide gel, the band of 324 bp was detected. This amplified region was specific for type A.
 
Keyword(s): BNYVV, DAS-ELISA, POLYMERASE CHAIN REACTION, TYPE A.
 
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